Nanion Corporate Blog

 

16.04.2018:  Maturation of iPSC - CM by pacing? Go for it! 

The CardioExcyte 96 is a hybrid system recording contractility, electrophysiology and viability of cardiomyocyte networks. No subtleties of cytotoxic responses are missed, measurements are performed at high resolution, are non-invasive and label-free.

In iPSC-derived cardiomyocytes, unlike adult CM, positive inotropic compounds like isoprenaline does not increase contraction force [1], which demonstrates the immaturity of this cell type. The lack of an inotropic reaction despite a pronounced chronotropic response after beta-adrenergic stimulation most likely indicates immaturity of the sarcoplasmic reticulum. Further maturation of cardiac cells can be achieved by continuous pacing over several weeks [2]. The CardioExcyte 96 platform allows for electrical and optical stimulation capabilities, long-term or short-term. Find out more here on the new sensor plates with pacing electrode.

[1] Pillekamp F et al. Contractile properties of early human embryonic stem cell-derived cardiomyocytes: Beta-adrenergic stimulation induces positive chronotropy and lusitropy but not inotropy. Stem Cells Dev. 2012; 10(21):2111–2121.

[2] Zhu R et al. Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes. Stem Cell Res Ther 2014; 5: 117

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