23.11.2018: New SyncroPatch 768PE paper out: comparison with manual patch clamp data
Vanoye et al, in the lab of Al George Jr. at Northwestern University, have been putting their SyncroPatch 768PE through its paces and have characterized 78 variants of the KCNQ1 channel using the instrument. Importantly the data agreed very well with manual patch clamp data. Indeed, normalized whole cell currents exhibited identical gating properties recorded using the two methods, displaying identical V1/2 of activation and inactivation. The group used electroporation to transfect cells with a high efficiency and using the SyncroPatch 768PE could characterize 48 variants in approximately 12 weeks and they estimate that 12 batches of KCNQ1 variants could be evaluated in as little as 2 weeks. Such high throughput elucidation of the functional consequences of channel variants provides new opportunities for precision medicine. Congratulations to all involved in this excellent paper.
Read more here.