26.05.2019: Replacing Radiolabeling Techniques With the SURFE2R N1 - a Case Study
Dr. Randy Stockbridge (Assistant Professor at the University of Michigan) was one of the two SURFE²R N1 Grant Winners from 2018 where they won SURFER N1 platform for 6-month period. We asked Randy about her experience, established assays and future plans with SURFE²R N1 instrument.
Dr. Randy Stockbridge's Research:
Membrane transport proteins – channels and energy-coupled pumps – are the molecular gatekeepers of the cell. For microbes subsisting in a hostile environment, these proteins import vital nutrients and export dangerous toxins.
Randy: "My lab is currently focused on discovering and understanding novel microbial membrane proteins. We are especially interested in fluoride channels, which protect bacteria against toxic environmental fluoride ion, and guanidinium ion exporters, which have an interesting evolutionary relationship to multidrug exporters involved in antibiotic resistance".
Dr. Randy Stockbridge about the SURFE²R Technology:
"We had done the initial characterization of our new family of transporters using radiolabeled substrate uptake, but we needed a more general assay in order to expand beyond commercially available radiolabeled substrates.
Now that we’ve started using the SURFE²R N1 instrument, this technique has replaced radiolabeled substrate experiments in my lab, to everyone’s benefit. We’re not spending as much money on radiolabeled substrates, the experiments are much faster to perform, with fewer regulatory headaches.“
Download the case study here.