Nanion Corporate Blog

 

04.11.2020: Patch Clamp Electrophysiology


Patch Clamp Electrophysiology (2020) - Chapter 4
Icon Orbit Orbit 16 and Icon Orbit Mini Orbit Mini - Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers

"We were delighted to be invited to write a chapter about protein purification and bilayer experiments on classical set-ups and also on the automated systems (Orbit 16 and Orbit mini) so that we can share our extensive knowledge about tips and tricks for bilayer measurements with a wider audience. The chapter describes some of the history behind bilayer experiments along with thorough protocols for protein purification through to fully automated bilayer recordings on 16 bilayers simultaneously and analysis of the data."

- Alison Obergrussberger, 2020
(Nanion Technologies) Co-Authored Chapter 4 in Patch Clamp Electrophysiology (2020)

This chapter focuses on protein purification and reconstitution of channel-forming proteins into lipid bilayers using a classic setup and on the commercially available systems, the Orbit mini and Orbit 16.

Download Chapter 4 here

Patch Clamp Electrophysiology (2020) - Chapter 7
icon pl Patchliner - Utilising Automated Electrophysiological Platform in Epilepsy Research

"An amazingly versatile platform for all your electrophysiological needs."

Carol Milligan, Excerpt from SelectScience Review of the Patchliner 2020
(The Florey Institute of Neuroscience and Mental Health) Co-Authored Chapter 7 in Patch Clamp Electrophysiology (2020)

In this chapter, the authors describe aspects of the Patchliner, e.g. examining the effects of temperature on stably and transiently transfected mammalian cells, the latter of which for most automated systems on the market is quite challenging. In addition, this chapter also showcases methods that demonstrate the versatility of the Patchliner and the Multi Channel Systems (MCS) multielectrode array (MEA) assay for acutely dissociated murine primary cortical neurons, enabling the study of potassium channel mutations implicated in severe refractory epilepsies.

Download Chapter 7 here


Patch Clamp Electrophysiology (2020) - Chapter 10
 icon pap Port-a-Patch  - Bioluminescence Methodology for Ion Channel Studies

"Automated planar electrophysiology could give us an advantage in the really early phase for discovery and screening, and for lead optimization."

- Fernanda LaezzaRecipent of SyncroPatch 384i Research Grant
Nanion Virtual User Meeting 2020
(University of Texas Medical Branch) Co-Authored Chapter 10 in Patch Clamp Electrophysiology (2020)

In this chapter, the authors describe the assay development steps to stably reconstitute the interaction between voltage-gated Na+ (Nav) channel Nav1.6 and its accessory protein, fibroblast growth factor 14 (FGF14) using the split-luciferase complementation assay (LCA), followed by assay miniaturization and optimization in 384-well plates for in-cell high-throughput screening (HTS) against protein-channel interactions. Additionally, the authors introduce the methodology for rapid functional hit validation studies using semi-automated planar patch-clamp electrophysiology (Port-a-Patch).

Download Chapter 10 here

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