02. - 06.02.2019 | SLAS 2019
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Meet our Ronald Knox, Leo Angelo Morada and Rodolfo Haedo in Washington DC at our booth #1614.
Meet our partners Genedata at booth #1317.
Recommended oral presentation:
Presenter: Tianbo Li, Scientist, Genentech
Title: "Development of High-throughput Data Analysis Methods to Bridge High-Throughput APC Assay with Ion Channel Research and Drug Discovery"
Date: Tuesday, February 5, 12:00 - 12:30, Room 147AB
Ion channels regulate a variety of physiological processes and represent the second largest class of known drug targets. Among the known methods to study ion channels, patch clamp electrophysiology remains the gold standard with its unsurpassable precision in all ion channel functional assays. The automated patch clamp (APC) screening technology has emerged to meet the challenge of scaling up this gold-standard method. However, due to the complexity of electrophysiological data, the fast-increasing APC throughput is facing a major challenge when it comes to robustly analyzing the data. Here I will discuss our recent breakthrough in this challenge by co-developing high-throughput screening data analysis methods with Genedata. To match our daily throughput of ~8,000 Nav channel recordings from the Nanion SyncroPatch 768PE, a data reduction stategy was used to reduce the original recording 1st level data size by ~1,000 times. Then the 2nd level data with more than 100,000 recordings can be analyzed by Genedata Screener® as one experiment. To ensure high data quality, an automated quality control method was developed by optimizing four key parameters: seal resistance, peak current, capacitance, and series resistance. Additionally, a customized SyncroPatch data analysis method was developed for Screener® to directly handle the 1st level raw data for kinetic characterization of Nav channel currents. Overall, the fast advancing APC high-throughput technologies together with the robust and high-throughput data analysis methods will have a significant impact on ion channel research and drug discovery.
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We are looking forward to seeing you in Washington DC!