CardioExcyte 96 – Combined contractility, electrophysiology and cell viability
The CardioExcyte 96 is a hybrid system recording both contractility and electrophysiology of intact cardiomyocyte networks. In addition, the base impedance is continuously and automatically monitored as a measure of acute and chronic cell activity. No subtleties of cytotoxic responses are missed, this includes non-contractile cell types such as hepatocyte-like cells or cancer cells as well as contractile cardiac cells. Impedance and extracellular field potential measurements are performed at high resolution, are non-invasive and label-free. The CardioExcyte 96 is a fully automated device, recording from 96 wells at a time. The benefits the CardioExcyte 96 offers include:
- Incubation system for hypoxic experimental conditions available
- FLEXcyte 96 add-on for contractility under physiological conditions
- Impedance & extracellular field potentials from the same well
- 96 recording wells in parallel
- Non-invasive & label-free measurements
- High resolution
- Electrical and optical pacing options
- Acute and chronic assessment of cell activity and toxicity
- Incubation system for hypoxic experimental conditions available
- Part of the CiPA validation study
With embedded electronics and a sophisticated sensor technology integrated in the well-plate based consumables, the CardioExcyte 96 is a turn-key system for efficient impedance and extracellular field potential measurements. The CardioExcyte 96 package includes an Incubation System which makes the system independent of an incubator. The system is placed on the bench top with maintained control over temperature, gas mix and humidity.
Efficiency and ease-of-use are facilitated by an outstanding software package for data handling and export - an essential part of the CardioExcyte 96 system. The system has been validated with stem cell-derived cardiomyocytes from all major stem cell providers, as monolayers or beating 3D-clusters, as well as with cell lines (hepatocyte-like cells, cancer cells etc.) for cytotoxicity studies.
For detailed information:
Add-Ons and Features
CardioExcyte 96 Platform
The CardioExcyte 96 package contains an Integra Viaflo Assist that provides automated multichannel pipetting for reproducible 96 well plate handling of cells and compounds.
CardioExcyte 96 Incubation System
The CardioExcyte 96 package includes an Incubation System. This means that the measurements are performed outside the cell culture incubator and the complete setup fits on a lab benchtop. The CardioExcyte 96 Incubation System controls temperature, gas mix and humidity. It is suitable for various experimental conditions (e.g. hypoxia).
CardioExcyte 96 SOL (Add-On)
Optical stimulation and investigation of impedance and extracellular field potentials in parallel:
The stimulating optical lid, CardioExcyte 96 SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2). After transfection of iPS cardiomyocytes with ChR2 the cells can be stimulated by blue light. Each light impulse induces a depolarization of the cells and thus an action potential is triggered. The advantages of optical stimulation over electrical stimulation include the highly precise timing, all cells of the beating network are stimulated exactly at the time of the light stimulus. In contrast, electrical stimulation propagates from the electrode across the well and thus cells are stimulated progressively. A mean beat calculation of precisely timed beats enables in-depth compound analysis and concentration response dependencies to be obtained. This is one of the key software capabilities of the CardioExcyte Control/ DataControl software package that is provided with each CardioExcyte 96 system.
Software
CardioExcyte 96 Software package
The CardioExcyte Control software supports online analysis of beating parameters. The unique Mean Beat Function automatically visualizes the average of beat traces from one well, enveloped by the standard deviation, indicating the consistency of the beat pattern within the recording well, i.e. if the cellular network is synchronously beating or not. The control software is intuitive and easy-to-use, at the same time very powerful in terms of experimental setup and online visualization of the recorded data.
The DataControl 96 software package is an independent software package to load and analyze data recorded on the CardioExcyte 96.The analysis template can be saved and reloaded. Customized export formats of analysis results are integrated.
Statement about the DataControl 96 Package
Hans-Peter Scholz - Statement about the Analysis Software of the CardioExcyte 96 "DataControl 96"
“DataControl 96 allows us to analyze large amounts of data and represents a huge step towards standardized experiment analysis. All the well's individual raw data, analysis results, concentration response curves and also the fits averaged per compound are displayed on a single screen, which means that all relevant information is right at the place where we want to see it. Analysis templates can be used to re-do the same analysis procedures on a different compound, but same target, thus reducing the effort for data handling to just a couple of mouse clicks. Powerful averaging with standard deviation display and referencing methods allow for great quality control. Furthermore, multiple export options are available to allow a seamless integration in databases.”
Hans-Peter Scholz, Technician,
Biopharma, Global Early Non Clinical Safety, Merck KGaA, Darmstadt, Germany
Consumables
NSP-96
The NSP-96 chip is a proprietary innovative product by Nanion Technologies, developed for the CardioExcyte 96. It is produced and quality-assured partly by an external partner and partly in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NSP-96 chips are available for different experiments.
Description and Material
- Standard 96-well plate format
- Working volume: 25-340 µl (surface 34mm2)
- Planar, bipolar circular gold electrodes
- Supports recordings from 2D and 3D structures
- Hybrid sensor design for both EFP and impedance recordings
Available chip types
- "NSP-96, CardioExcyte 96 Sensor Plates - Stim": Type standard with extra stimulation electrode (Order # 201003)
- "NSP-96 CardioExcyte96 Sensor Plates 0.6 mm": Type standard, 0.6 mm recording electrode (Order # 201002)
- "NSP-96 CardioExcyte96 Sensor Plates 2.0 mm": Type standard, 2.0 mm recording electrode (Order # 201001)
Other types are available upon request!
CardioExcyte 96 and 
FLEXcyte 96 product flyer on plates available 
(0.90 MB)
Testimonials & Case Studies
Prof. Bjorn C. Knollmann - Statement about the CardioExcyte 96
“We are happy to have acquired the CardioExcyte 96 from Nanion and look forward to publishing the first of many papers. We looked at several different devices on the market but were impressed by the resolution and ease-of-use of the CardioExcyte 96 and Nanion’s enthusiasm and friendly customer support. I was particularly intrigued by the pacing feature, the single electrode design giving better signal to noise ratio, the dual EFP + impedance capability, and the environmental chamber so that you can run everything on the benchtop. The new optical pacing feature added in 2017 has further expanded the utility of the instrument for drug testing and disease modeling: For the first time, rate-dependent changes of cardiac electrophysiology and contractility can be easily quantified.”
Prof. Bjorn C. Knollmann, Director of the Center for Arrhythmia Research and Therapeutics of Vanderbilt University School of Medicine:
Marc Rogers - Statement about the Patchliner and CardioExcyte 96
"It has been an absolute pleasure and privilege to work with Niels and the Nanion team over the past decade. I firmly believe that our two small, but independently minded ion channel focused companies, share a similar business ethos and a clear focus on delivering cost-effective solutions to our customers. In our extensive use of the Patchliner platform since 2007, my teams have benefited from the dedication of Nanion’s service engineers, application scientists, and bespoke data acquisition routines, whilst Nanion have benefited from seeing their products applied and adapted to real-world drug discovery projects and assays."
"This fruitful working relationship has now been extended to our use of the CardioExcyte96 phenotypic assay platform, which we utilise to develop and validate human iPSC cardiomyocyte cell lines and assays to meet the post-hERG, predictive cardiac safety testing needs of the drug discovery industry”."
Dr. Marc Rogers, CSO
Metrion Biosciences
Hans-Peter Scholz - Statement about the Analysis Software of the CardioExcyte 96 "DataControl 96"
“DataControl 96 allows us to analyze large amounts of data and represents a huge step towards standardized experiment analysis. All the well's individual raw data, analysis results, concentration response curves and also the fits averaged per compound are displayed on a single screen, which means that all relevant information is right at the place where we want to see it. Analysis templates can be used to re-do the same analysis procedures on a different compound, but same target, thus reducing the effort for data handling to just a couple of mouse clicks. Powerful averaging with standard deviation display and referencing methods allow for great quality control. Furthermore, multiple export options are available to allow a seamless integration in databases.”
Hans-Peter Scholz, Technician,
Biopharma, Global Early Non Clinical Safety, Merck KGaA, Darmstadt, Germany
Dr. Ralf Kettenhofen - Statement about the CardioExcyte 96
"CardioExcyte 96 is an easy-to-use system, providing impedance-based and MEA-like cardiac safety data from a diversity of stem cell-derived cardiomyocytes, and constitutes an excellent complement to automated patch clamp-based safety screening. Concentration- and time-dependence of a compound’s potential cardiotoxicity can efficiently be obtained where the alteration of beating patterns can give a hint as to which cardiac ion channel(s) is affected, after which detailed electrophysiology investigations can be undertaken. Cardiac network responses offer a comprehensive view of a compound’s safety profile, without having to use in-vivo methods, which saves time, costs and suffering. Further on, the powerful software, used for recordings and analysis, employs comprehensive beat investigation algorithms, displaying detailed beating kinetics in real-time. Data handling and export is straightforward, easy to grasp and yet very, very powerful."
Dr. Ralf Kettenhofen, Head of Laboratory
Ncardia, Cologne, Germany.
Dr. Daniel Konrad - Statement about the CardioExcyte 96
“Impedance and field potential data of beating cardiomyocytes is getting increasingly important to obtain a comprehensive view of the cardiac liability of test compounds. To answer these questions for our clients, B’SYS successfully implemented the CardioExcyte 96 system and provides related validated services to its customers, mostly using ipS derived cardiomyocytes. B’SYS’ scientists are pleased with the CardioExcyte’s ease of operation and data acquisition. The powerful software allows for applying meaningful data analysis and time saving export of data as needed for our daily customer reports.“
Dr. Daniel Konrad
CEO B'SYS
Anika Duenbostell-Schmidt - Statement about the CardioExcyte 96
"I use the CardioExcyte 96 weekly to test substances on our human iPSC-derived cardiomyocytes. It allows me to map, compare and evaluate a wide variety of pharmacological responses with a high degree of reliability and uniformity, and evaluate them through the rapid analysis function."
Anika Duenbostell-Schmidt, Head QC at Ncardia
Ncardia, Cologne, Germany.
Data and Applications
NCX1 - Effect of block of cardiac NCX on beat rate
CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.
Block of NCX1 expressed in iCell® Cardiomyocytes2 by SEA 0400 caused a dose dependent increase in beat rate.
Cardiac Ion Channels - Pharmacology of Vandetanib
CardioExcyte 96 and Patchliner data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.
The image on the left hand side displays the results of the blocking effect of Vandetanib on hERG, NaV1.5, CaV1.2 and KV4.3. The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 1 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility.
Cardiac Ion Channels - Pharmacology of Sotalol
CardioExcyte 96 and 
SyncroPatch 384PE (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.
The image on the left hand side displays the results of the blocking effect of Sotalol on hERG. The result is in good agreement with manual patch clamp data (Crumb et al., 2016). The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 10 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility measurements.
Cardiomyocytes - Data from different cell providers
CardioExcyte data and applications:
Cells were kindly provided by FUJIFILM Cellular Dynamics, Nexel, Ncardia, Axiogenesis, Pluriomic, GE Healthcare, ReproCell, Takara Bio Cellartis Clonetech.
The CardioExcyte 96 allows for
• Non-invasive, label-free measurements of beating cardiomyocyte networks
• 96 recording wells in parallel with 1 ms time resolution
• Quick experiments or long-term compound effects on cardiotoxicity
• Real-time access to beating parameters
• Outstanding software for data analysis and export
• Cost effcient consumables - 96-well format
Cardiomyocytes - Time-dependent effect of Pentamidine on Cor.4U cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Time-dependent effect of Pentamidine on Cor.4U cardiomyocytes. Pentamidine clinically causes acquired long QT syndrome, which is associated with prolonged QT intervals, tachycardias, and sudden cardiac arrest. Pentamidine delays terminal repolarization in human heart by acutely blocking cardiac inward rectifier currents. At the same time, it reduces surface expression of the cardiac potassium channel IKr/human ether à-go-go-related gene (hERG).
Insets: EFP raw traces and online analysis value FPDc in the presence of Pentamidine (10 µM) at different timepoints.
Cardiomyocytes - Tetracaine dose response curves as recorded with Cor.4U cells
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Impedance amplitude is not changed by addition of increasing concentrations of Tetracaine (left panel), while beat rate of Cor.4U® cells is decreasing. For example, 29.6µM of Tetracaine decreased the beat rate by ~60% when compared to pre-addition values. Cumulative dose-response relationships indicate Tetracaine potency for same-well additions. Representative raw traces for impedance signals (middle panel) clearly indicate a decrease in cell monolayer beat rate with increasing concentrations of Tetracaine.
Extracellular Field Potential (EFP) spike amplitude is decreased by cumulative Tetracaine dose applications to the same monolayer of Cor.4U® cardiomyocytes (top right), in agreement with compound mechanism of action. Representative raw traces for EFP signals (bottom graph) clearly indicate a decrease in spike amplitude.
Cardiomyocytes - Nifedipine and its concentration dependent effect on Cor.4U cells
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Nifedpinie is a dihydropyridine calcium channel blocker that primarily blocks L-type calcium channels. Impedance and EFP recordings on Cor.4U cells reveal a concentration dependent effect on impedance amplitude, beat rate and also a shortening of the FPD as expected.
Cardiomyocytes - Effect of E4031 on the impedance and EFP signals on iCell cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.
Effect of the specific hERG blocker, E4031, on the impedance and EFP signals. A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031 (300 nM, 3 mins incubation) induces EAD, shown in the inset, which can lead to potentially fatal ventricular arrhythmias. B EFP signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031(300 nM, 30 mins incubation) also causes arrhythmic effects in the EFP mode shown in the inset.
Cardiomyocytes - Effect of the ß-adrenergic receptor agonist, Isoproterenol, on the impedance and EFP signals on iCells
CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.
A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in isoproterenol at the concentrations indicated. Isoproterenol acts as a heart stimulant, it increases heart rate, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid. In the presence of even low concentrations of isoproterenol (1 nM), an increase in the beat rate is observed. B Bar graph showing beat rate before (dark blue) and after (light blue) incubation in isoproterenol at the concentrations indicated. At all concentrations, a clear increase in beat rate is observed. C EFP signal of 12 wells in control conditions (left) and the same 12 wells after 30 mins incubation in isoproterenol at the concentrations indicated (right). Isoproterenol shortens the FPD, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid.
Cardiomyocytes - Effects of verapamil on impedance (left) and EFP (right) signals of Cellartis Cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Takara Bio Cellartis Clontech.
(A) Impedance (left) and EFP signals (right) in control conditions
(B) Upon application of 10 nM verapamil
(C) Upon application of 100 nM verapamil.
(D) The mean beats for impedance (left) and EFP (right) are shown in D
Cardiomyocytes - Effects of sotalol on impedance (left) and EFP (right) signals of Cellartis Cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Takara Bio Cellartis Clontech.
Impedance (left) and EFP signals (right) in control conditions (A) and 1 μM sotalol (B) are shown.
Irregular beating can be observed in the presence of sotalol.
Murine Mammary Carcinoma Cells - Effect of CAF (cyclophosphamide, adriamycin (doxorubicin) and 5-fluouracil)
CardioExcyte data and applications:
Data kindly provided by Oliver Reinhardt, MPI Experimentelle Medizin, Göttingen
Murine mammary carcinoma cells (H8N8 and H8N8 T3.2) were monitored over a time period of 500 h. Changes in impedance, and therefore confluency, were used as a measure of toxicity.
H8N8 cells have been developed as a tool to investigate tumor cell recurrence, chemoresistance and epithelial-to-mesenchymal transition (EMT). Cells were treated with increasing concentrations of CAF (cyclophosphamide, adriamycin (doxorubicin) and 5-fluouracil) on the CardioExcyte 96 during impedance measurements. Therefore, the CardioExcyte 96 in combination with murine mammary carcinoma cells provides a novel tool for investigating therapy resistance of cancer cells in vitro.
Data kindly provided by Oliver Reinhardt, MPI Experimentelle Medizin, Göttingen
Cardiomyocytes - Optogenetics meets cardiac safety
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
The stimulating optical lid, CardioExcyte 96 SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2).
Right graph: LPM – Light pulse per minute plotted against the recorded beat rate (average of 96 wells). ChR2 transfected Cor.4U cells are following the optical pace rate.
Cardiomyocytes - Channelrhodopsin 2 (ChR2) transfected Cor.4U cells and optical pacing
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
ChR2 transfected Cor.4U cells are following the optical pace rate.
Raw data traces upon a 1 Hz, 1.5 Hz, 2 Hz., 2.5 Hz and 3 Hz stimulation rate, extracellular field potentials (top) and impedance (bottom).
Cardiomyocytes - Myocyte phase II study: CiPA conform analysis and arrhythmia detection
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Nanion developed a CiPA conform analysis for the Myocyte phase II study. The feature comes along is included in our CiPA analysis routine. Automated arrhythmia detection is just one highlight out of many when it comes to the CardioExcyte 96 software.
Hepatocytes - Paracetamol toxicity on hepatocyte-like MetaHeps cells
CardioExcyte data and applications:
Cells were kindly provided by MetaHeps.
The impedance signal recorded on the CardioExcyte 96 changes as a result of alterations in confluency, cell contact (morphological shape) and conductivity of adherent cells and thereby provides a measure of toxicity. MH cells from MetaHeps® were grown on NSP-96 plates and base impedance was monitored over time. Increasing concentrations of paracetamol induce a decrease in base impedance of MH cells which can be monitored continuously. Tween (2%) induced 100% cell death and was used as a positive control.
Webinars and Movies
Webinars
13.10.2020 | Webinar: Impedance measurements and Calcium imaging to investigate the role of store operated Ca2+ entry in GPCR-mediated endothelial barrier regulation
CardioExcyte 96 Webinar
Date: October 13. 2020
Speakers:
Dr. Judith Stolwijk (University Hospital Regensburg; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
13.10.2020 | Webinar: Profiling the pharmacology of G-Protein coupled receptors (GPCR) in cell-based assays using label-free impedance analysis
CardioExcyte 96 Webinar
Date: October 13. 2020
Speakers:
Michael Skiba (University Hospital Regensburg; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
15.10.2020 | Webinar: Heart slice culture system reliably demonstrates clinical drug-related cardiotoxicity
CardioExcyte 96 Webinar
Date: October 15. 2020
Speakers:
Dr. Tamer M. Mohamed (University of Louisville)
This is an on-demand webinar from Nan]i[on and Friends 2020.
17.03.2020 | Webinar: A new technology to evaluate mature cardiac contractility on high throughput level
FLEXcyte 96, CardioExcyte 96 Webinar
Date: March 17. 2020, 4:00 PM CET (11:00 AM EDT)
The Webinar focuses on the FLEXcyte 96 technology, an Add-on of the CardioExcyte 96 instrument. The analysis of cardiomyocyte contractility as well as data of short-termed and long-termed compound applications is discussed.
Speakers:
Dr. Sonja Stölzle-Feix
Dr. Matthias Gossmann
12.09.2018 | Webinar: CiPA study: Bridging ion channel and myocyte data
CardioExcyte 96, Patchliner and SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) Webinar
Date: September 12, 4:00 PM CEST (10:00 AM EDT)
Get up-to-date with the CiPA progress of the Myocyte and Ion Channel Work Goups:
- CiPA myocyte phase II validation study results: cross-site comparison using the CardioExcyte 96
- HTS Phase I study: an update on progress of the CiPA Ion Channel Work Stream using the SyncroPatch 384PE and Patchliner
28.04.2015 | Webinar: Excited About Contraction – Combining Contractility and Excitability Measurements in Cardiotoxicity Screening
CardioExcyte 96
This Webinar covers the introduction of the CardioExcyte 96, a hybrid device for impedance- and MEA-type recordings from intact, beating networks of stem cell-derived cardiomyocytes.
Movies: Tutorials and Oral Presentations
2020 - CardioExcyte 96 Tutorial Video
CardioExcyte 96
Short introduction of the CardioExcyte 96 (tutorial video, 2 minutes)
2018 - HTS Phase I study: an update on progress of the CiPA Ion Channel Work Stream using the SyncroPatch 384PE and Patchliner
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i), Patchliner and CardioExcyte 96 Oral Presentation
Presenter:
Tim Strassmaier, Nanion Technologies Inc. USA
Source:
Webinar: "CiPA study: Bridging ion channel and myocyte data", September 12, 2018
2018 - CiPA myocyte phase II validation study results: cross-site comparison using the CardioExcyte 96
CardioExcyte 96 Oral Presentation
Presenter:
Dr. Sonja Stölzle-Feix, Nanion Technologies
Source:
Webinar: "CiPA study: Bridging ion channel and myocyte data", September 12, 2018
2017 - Impedance-Based Monitoring of Cell-Based Assays: The Power of MultiFrequency Recordings
CardioExcyte 96 Oral Presentation
Presenter:
Prof. Dr. Joachim Wegener, University of Regensburg, Germany
15.10.2020 | Webinar: Heart slice culture system reliably demonstrates clinical drug-related cardiotoxicity
CardioExcyte 96 Webinar
Date: October 15. 2020
Speakers:
Dr. Tamer M. Mohamed (University of Louisville)
This is an on-demand webinar from Nan]i[on and Friends 2020.
13.10.2020 | Webinar: Profiling the pharmacology of G-Protein coupled receptors (GPCR) in cell-based assays using label-free impedance analysis
CardioExcyte 96 Webinar
Date: October 13. 2020
Speakers:
Michael Skiba (University Hospital Regensburg; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
13.10.2020 | Webinar: Impedance measurements and Calcium imaging to investigate the role of store operated Ca2+ entry in GPCR-mediated endothelial barrier regulation
CardioExcyte 96 Webinar
Date: October 13. 2020
Speakers:
Dr. Judith Stolwijk (University Hospital Regensburg; Germany)
This is an on-demand webinar from Nan]i[on and Friends 2020.
Downloads:
Application Notes
Cardiac Ion Channels - "High Throughput Screening of Cardiac Ion Channels"
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) Patchliner CardioExcyte 96 application note (2.3 MB)
Cardiomyocytes - "Chronic cardiotoxic effects of tyrosine kinase inhibitors and anthracyclines analyzed with the FLEXcyte 96 on human iPSC-derived cardiomyocytes"
FLEXcyte 96 application note (0.4 MB) kindly provided by InnoVitro GmbH
Cardiomyocytes - "Combining automated patch clamp, impedance and EFP of hiPSC-CMs"
CardioExcyte 96 SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) Patchliner Application Note
Cells kindly provided by Takara-Clonetech.
Cardiomyocytes - "Impedance and EFP recordings of Cor.4U cells using Nanion’s CardioExcyte 96"
CardioExcyte 96 Application Note (1.3 MB)
Cells were kindly provided by Ncardia.
Cardiomyocytes - "Impedance and EFP recordings of iCell Cardiomyocytes² on the CardioExcyte 96"
CardioExcyte 96 Application Note (2.8 MB)
Cells were kindly provided by Cellular Dynamics.
Cardiomyocytes - "Impedance and EFP recordings of Pluricyte Cardiomyocytes on the CardioExcyte 96"
CardioExcyte 96 Application Note (1.3 MB)
Cells were kindly provided by Ncardia.
Cardiomyocytes - "Positive inotropic effects in human iPSC-derived cardiomyocytes can be triggered through a flexible pro-maturation environment"
FLEXcyte 96 application note (0.3 MB) kindly provided by InnoVitro GmbH
Cardiotoxicity - "Assessing cardiotoxic risk of anti-cancer agents on Nanion’s CardioExcyte 96"
CardioExcyte 96 Application Note (0.6 MB)
Cells were kindly provided by Ncardia and experiments performed by Oliver Reinhardt and Dr. Frauke Alves from the University of Göttingen.
CytoSwitch - "Inducing cell death using a photostatin on the CardioExcyte 96 and SOL"
CardioExcyte 96 and SOL Application Note (0.4 MB)
Cells were kindly provided by LMU Munich/CytoSwitch.
Hepatocytes - "Investigating DILI using MetaHeps cells on Nanion’s CardioExcyte 96"
CardioExcyte 96 Application Note (0.7 MB)
Cells were kindly provided by Metaheps GmbH.
Hepatocytes - Toxicity analysis in expanded upcyte® liver cells on Nanion’s CardioExcyte 96
CardioExcyte 96 Application Note (7.8 MB)
Cells were kindly provided by upcyte technologies
hiPSC-CM - "Conduction velocity with the 2-electrode layout of the CardioExcyte 96"
CardioExcyte 96 Application Note (1.4 MB)
Cardiosight®-S hiPSC-CMs kindly provided by Nexel
Product Sheets
CardioExcyte 96 - Product Sheet
CardioExcyte 96 product sheet (2.6 MB)
CardioExcyte 96 / FLEXcyte 96 Product Flyer - Plates
CardioExcyte 96 and FLEXcyte 96 Product flyer (1.3 MB)
CardioExcyte 96 Flyer - Pacing
CardioExcyte 96 Product flyer (1.0 MB)
CardioExcyte 96 Flyer - SOL
CardioExcyte 96 Product flyer (2.0 MB)
The Cell Monitoring - Product Brochure
CardioExcyte 96 and FLEXcyte 96 Product brochure (3.5 MB)
Publications
2021 - Transient Cell Cycle Induction in Cardiomyocytes to Treat Ischemic Heart Failure
CardioExcyte 96 prepublication in Research Square (2021)
Authors:
Abouleisa R., Ou Q., Tang X-L., Solanki M., Guo Y., Nong Y., Mcnally L., Lorkiewicz P., Kassem K., Ahern B., Choudhary K., Thomas R., Huang Y., Juhardeen H., Siddique A., Ifthikar Z., Salama A-B., Hammad S., Elbaz A., Ivey K., Satin J., Hill B., Srivastava D., Bolli R., Mohamed T.
2021 - Towards engineering heart tissues from bioprinted cardiac spheroids
CardioExcyte 96 Publication in IOP Science (2021)
Authors:
Polonchuk L., Surija L., Lee M.H., Sharma P, Ming C.L.C., Richter F., Ben-Sefer E., Rad M.A., Sarmast H.M.S., Shamery W.A.,Tran H.A., Vettori L., Haeusermann F., Filipe E.C., Rnjak-Kovacina J., Cox T., Tipper J., Kabakova I., and Gentile C.
2021 - Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy
CardioExcyte 96 Publication in Cell & Development Biology (2021)
Authors:
Li B., Xiong W., Liang W-M., Chiou J-S., Lin Y-J., Chang A.C.Y.
2021 - Sex-Specific Control of Human Heart Maturation by the Progesterone Receptor
CardioExcyte 96 Publication in Circulation (2021)
Authors:
Sim C.B., Phipson B., Zienabb N., Rafehi H., Mills R.J., Watt K.I., Abu-Bonsrah K.D., Kalathur R.K.R., Voges H.K., Dinh D.T., ter Huurne M.,Vivien C.J., Kaspi A., Kaipanaickal H., Hidalgo A., Delbridge L.M.D., Robker R.L., Gregorevic P., dos Remedios C.G., Lal S., Piers A.T., Konstantinov I.E., Elliott D.A., El-Osta A., Oshlack A., Hudson J.E., Porrello E.R.
2021 - Overlap Arrhythmia Syndromes Resulting from Multiple Genetic Variations Studied in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
CardioExcyte 96 Publication in International Journal of Molecular Sciences (2021)
Authors:
Treat J.A., Pfeiffer R., Barajas-Martinez H., Goodrow R.J., Bot C., Haedo R.J., Knox R., Cordeiro J.M.
2021 - Long noncoding RNA VENTHEART is required for cardiomyocyte specification and function
CardioExcyte 96 pre-print publication in bioRxiv (2021)
Authors:
Dashi A., Tan W.L.W., Anene-Nzelu C.G., Pan B., Ilmari A.M., Tiang Z., Hartman R.J.G., Stenzig J., Wei H., Gao Bin C., Ackers-Johnson M.A., Lim B., Walentinsson A., Iyer V.V., Jonsson M.K.B., Foo R.S.
2021 - Hypoglycemia Induced Mitochondrial Connexin-43 Accumulation Aggravates Diabetic Cardiomyopathy
CardioExcyte 96 pre-publication in Research Square (2021)
Authors:
Wei X., Chang A.C.H., Chang H., Xu S., Xue Y., Zhang Y., Lei M., Chang A.C.Y., Zhang Q.
2021 - Cardiac Safety of Kinase Inhibitors – Improving Understanding and Prediction of Liabilities in Drug Discovery Using Human Stem Cell-Derived Models
CardioExcyte 96 prepublication in Frontiers in Cardiovascular Medicine (2021)
Authors:
Ziegler R., Häusermann F., Kirchner S., Polonchuk L
2021 - Anthracycline-Induced Cardiotoxicity: Molecular Insights Obtained from Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes (hiPSC-CMs)
CardioExcyte 96 Publication in The AAPS Journal (2021)
Authors:
Bozza W.P., Takeda K., Alterovitz W-L., Chou C-K., Shen R-F., Zhang B.
2020 - Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation
CardioExcyte 96 publication in Nature Communications (2020)
Authors:
Wang Y., Yao F., Wang L., Li Z., Ren Z., Li D., Zhang M., Han L., Wang S., Zhou B., Wang L.
2020 - Molecular Profiling of Human Induced Pluripotent Stem Cell-Derived Cells and their Application for Drug Safety Study
CardioExcyte 96 Review in Current Pharmaceutical Biotechnology (2020)
Authors:
Matsui T., Miyamoto N., Saito F., Shinozawa T.
2020 - Metformin therapy confers cardioprotection against the remodeling of gap junction in tachycardia-induced atrial fibrillation dog model
CardioExcyte 96 publication in Frontiers in Life Sciences (2020)
Authors:
Li J., Li B., Bai F., Ma Y., Liu N., Liu Y., Wang Y., Liu Q.
2020 - Maurocalcin and its analogue MCaE12A facilitate Ca2+ mobilization in cardiomyocytes
CardioExcyte 96 publication in Biochemical Journal (2020)
Authors:
De Waard S., Montnach J., Cortinovis C., Chkir O., Erfanian M., Hulin P., Gaborit N., Lemarchand P., Mesirca P., Bidaud I., Mangoni M., De Waard M., Ronjat M.
2020 - Functional Impact of BeKm-1, a High-Affinity hERG Blocker, on Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells
CardioExcyte 96 publication in Molecular Sciences (2020)
Authors:
De Waard S., Montnach J., Ribeiro B., Nicolas S., Forest V., Charpentier F., Mangoni M.E., Gaborit N., Ronjat M., Loussouarn G., Lemarchand P., De Waard M.
2020 - Elevated myocardial SORBS2 and the underlying implications in leftventricular noncompaction cardiomyopathy
CardioExcyte 96 publication in EBioMedicine (2020)
Authors:
Li C., Liu F., Liu S., Pan H., Du H., Huang J., Xie Y., Li Y., Zhao R., Wei Y.
2020 - Comprehensive Cardiac Safety Assessment using hiPS-cardiomyocytes
CardioExcyte96 publication in the Journal of Current Pharmaceutical Biotechnology (2020)
Authors:
Takasuna K., Kazusa K., Hayakawa T.
2020 - Advancing physiological maturation in human induced pluripotent stem cell‐derived cardiac muscle by gene editing an inducible adult troponin isoform switch
CardioExcyte 96 publication in STEM CELLS (2020)
Authors:
Wheelwright M., Mikkila J., Bedada F.B., Mandegar M.A., Thompson B.R., Metzger J.M.
2020 - A Scalable Approach Reveals Functional Responses of iPSC Cardiomyocyte 3D Spheroids
CardioExcyte 96 publication in SLAS Discovery (2020)
Authors:
Burnham M.P., Harvey R., Sargeant R., Fertig N., Haddrick M.
2019 - Transcriptomic profiling reveals p53 as a key regulator of doxorubicin-induced cardiotoxicity
CardioExcyte 96 publication in Cell Death Discovery (2019)
Authors:
McSweeney K.M., Bozza W.P., Alterovitz W.-L., Zhang B.
2019 - The patient-independent human iPSC model – a new tool for rapid determination of genetic variant pathogenicity in long QT syndrome
CardioExcyte 96 publication in Heart Rhythm (2019)
Authors:
Chavali, N.V., Kryshtal, D.O., Parikh, S.S., Wang, L., Glazer, A.M., Blackwell, D.J., Kroncke, B.M., Shoemaker, M.B., Knollmann, B.C.
2019 - The N-termini of GRK2 and GRK3 simulate the stimulating effects of RKIP on β-adrenoceptors
CardioExcyte 96 publication in Biochemical and Biophysical Research Communications (2019)
Authors:
Maimari T., Krasel C., Bünemann M., Lorenz K.
2019 - Pharmacological enhancement of repolarization reserve in human induced pluripotent stem cells derived cardiomyocytes
CardioExcyte 96 publication in Biochem. Pharmacol. (2019)
Authors:
Treat JA, Goodrow RJ, Bot CT, Haedo RJ, Cordeiro JM.
2019 - Particulate matter 2.5 induced arrhythmogenesis mediated by TRPC3 in human induced pluripotent stem cell-derived cardiomyocytes
CardioExcyte 96 publication in Archives of Toxicology (2019)
Authors:
Cai C., Huang J., Lin Y., Miao W., Chen P., Chen X., Wang J., Chen M.
2019 - Label-Free Monitoring of Cells in vitro
CardioExcyte 96 book chapter in Label-Free Monitoring of Cells in vitro in Bioanalytical Reviews - Springer (2019)
Authors:
Stolwijk J.A., Wegener J.
2019 - Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib
CardioExcyte 96 publication in Frontiers in Pharmacology (2019)
Authors:
Shen L., Li C., Zhang H., Qiu S., Fu T., Xu Y.
2019 - An integrated characterization of contractile, electrophysiological, and structural cardiotoxicity of Sophora tonkinensis Gapnep. in human pluripotent stem cell-derived cardiomyocytes
CardioExcyte 96 publication in Stem Cell Research & Therapy (2019)
Authors:
Wang R., Wang M., Wang S., Yang K., Zhou P., Xie X., Cheng Q., Ye J., Sun G., Sun X.
2018 - The opioid oxycodone use‐dependently inhibits the cardiac sodium channel Nav1.5
CardioExcyte 96 publication in British Journal of Pharmacology (2018)
Authors:
Meents J.E., Juhasz K., Stölzle-Feix S., Peuckmann-Post V., Rolke R. Lampert A.
2018 - Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
CardioExcyte 96 publication in Stem Cells and Development
Authors:
Kondrashov A., Hoang M.D., Smith J., Bhagwan J., Duncan G., Mosqueira D., Munoz M., Vo N.T.N., Denning C.
2018 - Safety Pharmacology Methods and Models in an Evolving Regulatory Environment
CardioExcyte 96 & CiPA Editorial in Journal of Pharmacological and Toxicological Methods (2018)
Authors:
Pugsley M.K., Harter M.L., de Korte T., Connaughton C., Authier S., Curtis M.J.
2018 - Hypertrophic cardiomyopathy-linked mutation in troponin T causes myofibrillar disarray and pro-arrhythmic action potential changes in human iPSC cardiomyocytes
CardioExcyte 96 publication in Journal of Molecular and Cellular Cardiology
Authors:
Wang L., Kim K., Parikh S., Cadar A.G., Bersell K.R., He H., Pinto J.R., Kryshtal D.O., Knollmann B.C.
2018 - Cross-site comparison of excitation-contraction coupling using impedance and field potential recordings in hiPSC cardiomyocytes
CardioExcyte 96 publication in Journal of Pharmacological and Toxicological Methods (2018)
Authors:
Bot C.T., Juhasz K., Haeusermann F., Polonchuk L., Traebert M., Stölzle-Feix S.
2017 - Structural and electrophysiological dysfunctions due to increased endoplasmic reticulum stress in a long-term pacing model using human induced pluripotent stem cell-derived ventricular cardiomyocytes
CardioExcyte 96 publication in Stem Cell Research and Therapy (2017)
Authors:
Cui C., Geng L., Shi J., Zhu Y., Yang G., Wang Z., Wang J., Chen M.
2017 - Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation
CardioExcyte 96 publication in International Journal of Molecular Sciences
Authors:
Rehnelt S., Malan D., Juhasz K., Wolters B., Doerr L., Beckler M., Kettenhofen R., Bohlen H., Bruegmann T., Sasse P.
2017 - Combined Impedance and Extracellular Field Potential Recordings from Human Stem Cell-Derived Cardiomyocytes
CardioExcyte 96 book chapter in Stem Cell-Derived Models in Toxicology (2017)
Authors:
Obergrussberger A., Thomas U., Stölzle-Feix S., Becker N., Juhasz K, Doerr L., Beckler M., George M., Fertig N.
2017 - An impedance-based approach using human iPSC-derived cardiomyocytes significantly improves in vitro prediction of in vivo cardiotox liabilities
CardioExcyte 96 publication in Toxicology and Applied Pharmacology (2017)
Authors:
Koci B., Luerman G., Duenbostell A., Kettenhofen R., Bohlen H., Coyle L., Knight B., Ku W., Volberg W., Woska Jr. J.R., Brown M.P.
2016 - Safety pharmacology studies using EFP and impedance
CardioExcyte 96 publication in Journal of Pharmacological and Toxicological Methods (2016)
Authors:
Obergrussberger A., Juhasz K., Thomas U., Stölzle-Feix S., Becker N., Dörr L., Beckler M., Bot C., George M., Fertig N.
2016 - Identification of Drug-Drug Interactions In Vitro: A Case Study Evaluating the Effects of Sofosbuvir and Amiodarone on hiPSC-Derived Cardiomyocytes
CardioExcyte 96 publication in Toxicological Sciences (2016)
Authors:
Millard DC, Strock CJ, Carlson CB, Aoyama N, Juhasz K, Goetze TA, Stoelzle-Feix S, Becker N, Fertig N, January CT, Anson BD, Ross JD.
2015 - Novel screening techniques for ion channel targeting drugs
Patchliner, SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and CardioExcyte 96 publication in Channels (2015)
Authors:
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.
2015 - New Easy-to-Use Hybrid System for Extracellular Potential and Impedance Recordings.
CardioExcyte 96 publication in Journal of Laboratory Automation (2015)
Authors:
Doerr L., Thomas U., Guinot D.R., Bot C.T., Stoelzle-Feix S., Beckler M., George M., Fertig N.
Posters
2021 - Cell Monitoring using Multi-Frequency Impedance Recordings for Label-free and Time-resolved Cell Response Analysis
CardioExcyte 96 poster, Presented at Safety Pharmacology Society Virtual Meeting 2020 and Society of Toxicology Annual Meeting 2021 (1.6 MB)
2019 - High content in vitro cell monitoring effects of adjuvant chemotherapy in breast cancer and cancer treatment-related cardiomyopathy
CardioExcyte 96 poster, SOT Conference 2019 (0.8 MB)
2018 - Combining electrophysiology and contractility recordings for more complete assessment of hiPSC-CMs
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384), Patchliner and 
CardioExcyte 96 poster, Europhysiology Meeting 2018 (1.4 MB)
2018 - A Sensor Based Technique for Pharmacological Safety Testing of Cardiac Transport Proteins NCX, NaKATPase and Respiratory Chain Complexes
CardioExcyte 96 and 
SUFE²R 96SE poster, SPS 2018 (1.7 MB)
2018 - Assessment of Drug Effects on Cardiomyocyte Function: Comprehensive In Vitro Proarrhythmia Assay (CiPA) Results
CardioExcyte 96 poster, SOT Meeting 2018 (1.8 MB)
2018 - Optogenetic technologies enable high throughput ion channel drug discovery and toxicity screening
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) and CardioExcyte 96 poster, Biophysics Annual Meeting 2018 (1.3 MB)
2018 - Investigations into idiosyncratic drug-induced hepatotoxicity and chronic proliferation of cancer cells using a label-free method
CardioExcyte 96 poster, Biophysics Annual Meeting 2018 (1.3 MB)
2016 - Next level toxicity screening: From single channel to overall cell behavior
Orbit mini, CardioExcyte 96 and SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, Meeting of the French Society of Toxinology (SFET) 2015 (0.9 MB)
2015 - Complementary automated patch clamp, extracellular field potential and impedance recordings of iPSCs: safety screening tool box for the future
Patchliner and CardioExcyte 96 and SyncroPatch 384PE (a predecessor model of SyncroPatch 384) poster, SPS 2015 (2.7 MB)
Presentations
2020 - Validation of a impedance-based phenotypic screening assay able to detect multiple mechanisms of chronic cardiotoxicity in human stem cell-derived cardiomyocytes
CardioExcyte 96 presentation (slide deck) (4.5 MB)
2018 - Optogenetic control of transiently transfected hiPSC-derived cardiomyocytes for the assessment of drug related cardiotoxicity
CardioExcyte 96 presentation (slide deck) (1.4 MB)
2018 - Innovations for cell monitoring in safety and toxicity assays
CardioExcyte 96 & SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) presentation (slide deck) (3.2 MB)
2017 - Cross-site comparison of myocyte phase II compounds on different iPS cardiomyocytes based on CardioExcyte 96 recordings
CardioExcyte 96 presentation (slide deck) (2.2 MB)
2017 - HTS in Cardiac Safety
CardioExcyte 96 presentation (slide deck) (4.0 MB)
