2017 - Impedance-Based Monitoring of Cell-Based Assays: The Power of MultiFrequency Recordings
CardioExcyte 96 Oral Presentation
Prof. Dr. Joachim Wegener, University of Regensburg, Germany
Nanion User Meeting
October 25.-26., 2018
Cell-based assays are a major experimental tool in drug development, fundamental biomedical research and also in judging environmental hazards. Besides having an appropriate cell culture model (e.g. cell line, primary culture, stem cells) for the problem under study, it is equally important to have sensitive tools capable of monitoring the cells during the experiment and reporting on the biological response. Within the last decades label-free physical approaches beyond light microscopy haven proven their usefulness and demonstrated unique advantages as they usually operate in a non-invasive, integrative manner and provide an unbiased, time-resolved perspective on the cells. Among these, impedance-based approaches belong to the key technologies. In impedance-based cellular assays adherent cells are grown on film electrodes - most often made from gold. Readings of the electrode impedance report either on changes in electrode coverage (e.g. during attachment or proliferation) or on morphological changes of the cells along the experiment. Based on these two fundamental sensitivities many different assays have been designed capable of probing, for instance, acute cytotoxicity, cell proliferation, cell differentiation, chemotaxis or signal transduction, to name just a few. The key to realize such a diverse list of applications based on rather generic bioanalytical readouts like electrode coverage and cell shape is (i) electrode design and (ii) the AC frequency that is used for impedance readings.
Joachim Wegener; Institut für Analytische Chemie, Chemo- & Biosensorik, Universität Regensburg (G); Fraunhofer EMFT, München (G)