• CE Slide 1
  • CE Slide 2
  • CE slide 3
  • CE slide 4
  • CardioExcyte 96

    Combined impedance and MEA-like recordings
  • CardioExcyte 96

    For cardiac safety screening
  • CardioExcyte 96

    Next generation label-free cell analysis
  • CardioExcyte 96

    Intuitive data analysis & arrhythmia detection

13.10.2020 | Webinar: Impedance measurements and Calcium imaging to investigate the role of store operated Ca2+ entry in GPCR-mediated endothelial barrier regulation

Icon CE  CardioExcyte 96 Webinar

Date: October 13. 2020

201012 Blog Image VUM 2020

Speakers:

Dr. Judith Stolwijk (University Hospital Regensburg; Germany)


This is an on-demand webinar from Nan]i[on and Friends 2020.

Abstract:

The endothelium of our blood vessels is under the control of various G protein-coupled receptors (GPCRs), regulating endothelial barrier function, vascular tone, angiogenesis and inflammation. Some of these GPCRs signal via Gq,11 protein, which activates Ca2+-release from the IP3-sensitive internal stores of the endoplasmic reticulum (ER). Calcium store depletion can subsequently activate so-called store-operated Ca2+ entry (SOCE) via the ER-resident Ca2+-sensor stromal-interacting molecule 1 (STIM1) and Orai1 Ca2+ entry channels.

We used Impedance measurements and ratiometric Calcium imaging to investigate the role of calcium entry via Orai1 channels downstream of barrier-regulating GPCRs in response to their agonists Thrombin, Histamine and Sphingosine-1-Phosphate (S1P) in human endothelial cells.


Back to Overview

 

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.