Orbit 16 - Taking the Pain out of Painting
Nanion's Orbit 16 platform takes the pain out of bilayer painting and subsequent recordings as it enables fast and successful data generation thanks to the rapid automated formation of 16 bilayers at once and their subsequent fully parallel recording. The benefits the Orbit 16 offers include:
- Automated formation of 16 artificial lipid bilayers at the push of a button
- Fully parallel low noise, high bandwidth recordings
- Substantial data generation due to 16 simultaneous recordings
- Low noise 16 channel amplifier or integration of external amplifier(s) possible
- Dedicated comprehensive recording software included
- Target molecules can be introduced directly or by fusion of (proteo-)liposomes
- Ion channels: voltage and ligand gated, temperature sensitive
- Nanopores, antimicrobial peptides, toxins and many more...
- Cost-efficient experiments with disposable MECA 16 chips
The Orbit 16 is equipped with a dedicated low-noise 16 channel amplifier (Elements s.r.l.) by default enabling the convenient fully parallel recording of 16 bilayers at highest bandwidths. Furthermore, most commercially available amplifiers are compatible with the Orbit 16, including the 16-channel Triton amplifier from Tecella. For single channel amplifiers like the EPC-10 or Axopatch, the Orbit has a built-in multiplexer switchboard, allowing for recordings from the individual bilayers by automatically switching through the recording sites.
The Orbit 16 bilayer platform
A complete Orbit 16 setup consists of the main recording station, an integrated Elements e16 bilayer amplifier and a recording module supporting Ionera's MECA 16 recording chips.
For detailed information:
Orbit 16 EDR Software
The software for the Orbit 16 was developed by our partner Elements SRL (Italy). It is intuitive and easy to learn. Please watch this video on the Orbit mini (contains the same software package as the Orbit 16) to learn about the software:
The MECA 16 recording substrate contains a 4 x 4 array of circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is automatically formed by remotely actuated painting (Ionera1-SPREAD), thus roofing the liquid-filled cavity. The bilayers can be easily and repeatedly zapped and re-formed in an automated fashion. After bilayer formation, ion channels or nanopores are reconstituted via self-insertion, proteoliposome fusion or dilution from detergent micelles.
The MECA recording chips are produced and quality assured by our partner Ionera Technologies GmbH in Freiburg Germany and shipped from Munich to our international customers. Different types of MECA 16 chips are available depending on the sample.
Available chip types
- "Meca 16 Recording Chips 50 µm": 16-well recording chip with 50 µm cavity size (Order # 131002)
- "Meca 16 Recording Chips 16 µm": 16-well recording chip with 16 µm cavity size (Order # 131003)
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