Alpha hemolysin - Lipid bilayer recordings of Alpha hemolysin using the Orbit 16 using the Micro Electrode Cavity Arrays (MECAs) chips
Orbit 16 application note (0.4 MB)
Bilayer recording is a well-established technique for in-depth studies of biophysical properties of ion channels and is particularly suited for functional studies on proteins residing in intracellular membranes. Moreover, this technique supports a host of powerful emerging analytical techniques using biological nanopores as molecular sensors. Despite its proven value, bilayer recording can be very frustrating due to the capricious nature of lipid bilayers, which have to be formed manually one by one and which often lack stability. We here show a new approach and device, which speeds up the entire process by the rapid and simultaneous formation of 16, highly stable micrometer-sized bilayers using microelectrode cavity array (MECA) chips. A study will be presented showing that the MECA supports high-resolution polymer sizing with a single biological nanopore in a parallel format.