• Orbit mini

    Simultaneous recording from four lipid bilayers

28.06.2018 | Webinar: Artificial Lipid Bilayers in focus: Hand-held DNA-sequencing and biosensing with nanopores

180628 event image webinar Orbit Icon Orbit Mini   Orbit mini and   Icon Orbit   Orbit 16

Learn about single channel measurements in bilayer recording using the orbit instrument family

- Simplifying artificial bilayer experiments: Single-molecule experiments on micro-cavity arrays
- Hand-held DNA-sequencing and biosensing with nanopores 

Speaker 1:
Dr. Conrad Weichbrodt
Nanion Technologies Germany

Simplifying artificial bilayer experiments: Single-molecule experiments on micro-cavity arrays

Artificial lipid bilayers represent the gold standard for the investigation of membrane spanning species like purified ion channels or membrane-active species in general like toxins. However, the convenient and reproducible preparation of these model bilayers as well as the delicate successive handling for data acquisition still remain an obstacle for the trouble-free introduction of this technique. We here present Nanion’s Orbit systems that are explicitly designed to meet the special requirements of experiments on artificial bilayers. Use of Ionera’s MECA (micro electrode cavity array) chip technology combined with state of the art low noise amplifiers (Elements S.R.L.) enables the fully parallel low-noise recording of four or even 16 separate lipid bilayers at bandwidths up to 100 kHz. The systems have already been validated with targets as diverse as ligand and voltage gated ion channels, porins and origami DNA constructs, antimicrobial peptides or membrane active toxins. The optional temperature control for the Orbit mini furthermore allows for experiments on temperature sensitive species such as TRP channels or for experiments at physiological temperatures whereas the fully automated bilayer generation on the Orbit e16 further improves the system’s usability

Speaker 2: 
Prof. Dr. Stefan Howorka, 
University College London, Department of Chemistry

Hand-held DNA-sequencing and biosensing with nanopores

Portable DNA sequencing and biosensing can advance research, bedside-diagnostics, and homeland security. I describe how label-free sensing is achieved with atom-scale designed membrane nanopores. In this strategy, nanopores act as electronic sensors that detect when individual molecules pass the pores’ nanoscale hole. The temporary blockages cause changes in ionic pore current. The approach has helped pioneer portable DNA sequencing with protein pores(1) to discriminate individual bases. More recently, synthetic pores have been built by folding DNA strands into defined channels(2). The DNA nanopores are relevant as they overcome the narrow size range of protein pores and thereby accommodate folded protein analytes. The DNA nanostructures are also easier to rationally design than proteins(3) and thereby enable new applications, also in synthetic biology(4). 

(1) Nature 2014 516 250;
(2) Nat. Nanotechnol. 2016 11 152;
(3) Nat. Nanotechnol. 2017 12 619;
(4) Science 2016 352 890; Nat. Chem. 2017 9 611

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