2015 - Orbit mini Membrane Preparation Tutorial Video
Short introduction how to prepare membranes on the Orbit mini
Manually paint a lipid bilayer on the chip’s four cavities by gently spreading lipid solution over them with e.g. one of the provided tools for painting (brush, Teflon piece in holder, glass rod)
Nanion recommends to paint bilayers via lipid covered air bubbles:
- Take e.g. a 10 μl Eppendorf pipette (small white tip).
- Cover the tip with lipids by dipping it into the lipid stock solution without aspirating any of it. Due to capillary forces a small amount of lipid solution will remain in the pipette tip - squeeze out any of these residues from the pipette tip.
- Place the tip on the chip’s surface close to a cavity, dispense a small air bubble (2-3 μl) over the cavity and aspirate it back. This results in the formation of a lipid bilayer which can be monitored by following the change in the current response: the signal should not go into saturation anymore and exhibit capacitive properties as shown in detail in the quickstart guide.
Repeat step three on all cavities. If bilayer formation is not successful within a first run start again at step one.
Note: Do not throw the tip away afterwards. It can be still used to repaint broken bilayers during the experiment.