Acetylcholine Receptor Alpha 7 - "Human a7 nicotinic Acetylcholine Receptor on the Patchliner "
Patchliner application note: (0.2 MB)
Cells were kindly provided by Galantos.
The human a7 nicotinic acetylcholine receptor expressing cells (stable) from Galantos Pharma GmbH were tested on the Patchliner. Repeated stimulations with 100 μM nicotine were tested to validate the system. In a run, six out of eight cells could be measured. All six cells showed characteristic a7 currents. Average peak currents for the shown experiment were 0.9 ± 0.2 nA (n = 6) which is consistent with manual patch clamp results reported by Galantos Pharma.