Cardiomyocytes - "Voltage and current clamp recordings of Cellartis hiPS-CM on Nanion’s Patchliner"
Patchliner application note: (0.5 MB)
Cells were kindly provided by Takara Bio Europe AB.
Human induced pluripotent stem (iPS) cell-derived cardiomyocytes have the potential to provide the ultimate model system for identifying potential anti-arrythmic effects of drugs during routine safety screening. Takara Bio Europe AB is providing a human iPS cell-derived cardiomyocyte product line for use in testing the efficacy and safety of pharmaceutical therapies. The ability to characterize the ion channel profile of these cells and record action potentials at a reasonable throughput is essential to fully realise the potential of this kind of product line. Building on the success of recording stem cells on the Patchliner, Cellartis® hiPS-CM, human iPS cell-derived cardiomyocytes, have now been characterized on the Patchliner in the voltage and current clamp mode.
In this Application Note we present data using an 8-channel Patchliner. In the voltage clamp mode, voltage-dependent Na+ (NaV), K+ (KV) and Ca2+ (CaV) channel currents were recorded. As expected, action potentials could be elicited in the current clamp mode. Furthermore, spontaneous action potentials could be recorded as well.