Patchliner

Most versatile automated patch clamp system on the market
Patchliner

In-house production and QC of consumables
Patchliner

More than 10 years experience in assay design and support
Dynamite⁸

Automated Dynamic Clamp
Patchliner

All features & benefits of manual patch clamp

2018 - Igor Tutorial for Patchliner Data Analysis

Patchliner tutorial

Presenter:
Dr. Nadine Becker, Product Manager Patchliner, Nanion Technologies GmbH, Germany

Content:

This Igor video tutorial for Patchliner data analysis demonstrates how concentration-response curves are quickly displayed and analyzed. We show how data are loaded and displayed, and the normalized data are fitted and averaged to obtain IC50 values. Please note that more details and features are described in our QuickStart Guide for Igor.

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Patchliner Applications

Whole cell current responses of HEK293 cells expressing Na_V1.7 to increasing voltage steps from -60 mV to 50 mV with corresponding average IV curve.
Dynamite⁸ (dynamic clamp) used to model I_K1 on action potentials of hiPSC cardiomyocytes.
TRPV1 expressed in CHO cells activated by increasing temperature (top). TRPV1 activated by capsaicin or heated solution in the same cell (bottom).
Fast solution exchange and minimal exposure ensures reproducible recordings of nAChαR expressed in HEK cells and repetitive exposure to 100 μM nicotine.
hK_Ca3.1 (SK4) expressed in CHO cells was activated by internal application of Ca²⁺.