Patchliner

Most versatile automated patch clamp system on the market
Patchliner

In-house production and QC of consumables
Patchliner

More than 10 years experience in assay design and support
Dynamite⁸

Automated Dynamic Clamp
Patchliner

All features & benefits of manual patch clamp

NaV1.5 - "Characterization of CreaCell's hNaV1.5 (A-0822) on Nanion's Patchliner"

Patchliner application note: (0.9 MB)
Cells were kindly provided by CreaCell.

Summary:

In this Application Note we present data characterizing hNa_V1.5 overexpressing HEK293 cells. The data were collected with Nanion‘s Patchliner. The performance of the cells was very good on the Patchliner. Current responses of an individual cell expressing the hNa_V1.5 channel to an IV voltage protocol are shown as well as the corresponding current-voltage relationship. The average mean peak current density of the cells was -215.1 ± 63 pA/pF at 0 mV (n=9).

Back to Overview

Subscribe to Our Newsletter/ e-mail Announcements

Email address

First Name

Last Name

News

Find a Distributor

Request a Quote

Schedule a Demo

Technical Support

Contact

Patchliner Applications

Whole cell current responses of HEK293 cells expressing Na_V1.7 to increasing voltage steps from -60 mV to 50 mV with corresponding average IV curve.
Dynamite⁸ (dynamic clamp) used to model I_K1 on action potentials of hiPSC cardiomyocytes.
TRPV1 expressed in CHO cells activated by increasing temperature (top). TRPV1 activated by capsaicin or heated solution in the same cell (bottom).
Fast solution exchange and minimal exposure ensures reproducible recordings of nAChαR expressed in HEK cells and repetitive exposure to 100 μM nicotine.
hK_Ca3.1 (SK4) expressed in CHO cells was activated by internal application of Ca²⁺.