TRPM8 / TRPV1 - "TRPV1 and TRPM8 recorded on Nanion's Port-a-Patch"
Port-a-Patch application note: (0.2 MB)
TRPV1 and TRPM8 are members of the transient receptor potential channel (TRP) family. This family was designated TRP because of a spontaneously occurring Drosophila mutant lacking TRP that responded to a continuous light with a transient receptor potential. TRPV1 is mainly expressed in sensory nerves. Its presence is essential for transduction of nociception as well as inflammatory and hypothermic effects of vanniloid compounds. It also contributes to acute thermal nociception and thermal hyperalgesia following tissue injury. TRPV1 forms a relatively Ca2+-selective ion channel with outwardly rectifying properties. It is activated by vanilloids such as capsaicin, by protons, increased temperatures, lipoxygenase products, as well as anandamide (Huang et al., 2002). Menthol, a secondary alcohol produced by the peppermint herb, Mentha piperita, is widely used in the food and pharmaceutical industries as a cooling/soothing compound and odorant. It induces Ca2+ influx in a subset of sensory neurons from dorsal root and trigeminal ganglia, due to activation of TRPM8, a Ca2+-permeable, cold-activated member of the TRP superfamily of cation channels (McKemy et al., 2002; Peier et al., 2002). Here we present data of TRPV1 and TRPM8 collected on the Port-a-Patch. Channel activation with capsaicin as well as menthol are shown.