• Port-a-Patch

    Smallest patch clamp setup in the world
  • Port-a-Patch

    Easy to learn - ideal for teaching
  • Port-a-Patch

    Records from cells, organelles and bilayers
  • Port-a-Patch

    First planar patch clamp device on the market
  • Port-a-Patch

    Ideal for internal solution exchange applications

Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"

icon pap   Port-a-Patch application note:   logo pdf   (0.5 MB) 

 Summary:

Bacterial membranes are not easy to patch clamp. Since bacterial ion channels are of increasing interest, we started to optimize the protocols for patch clamping bacterial spheroplasts with the Port-a-Patch. Bacterial spheroaplasts can be prepared up to a size of 5 µm. They consist of the inner bacterial membrane. This technique was first used for patch clamp experiments by Boris Matrinac in 1987 and let to the discovery of mechanosensitive channels in E. coli. Here we describe the preparation of Spheroplasts out of E. coli and show ion channel currents recorded with the Port-a-Patch.

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