TRPM8 - Cold activation of TRPM8 using the External Perfusion System and the Port-a-Patch
Port-a-Patch application note: (3.63 MB)
TRPM8 is a member of the transient receptor potential channel (TRP) family. TRPM8 is known to be a thermosensitive channel, activated by cold temperatures (below ~25˚C) and ligands such as menthol, Eucalyptol and icilin1-4. It belongs to the melastatin subfamily of TRP channels5 and shows an outward rectification with a relatively high permeability for calcium ions and little selectivity between monovalent cations. Menthol, a secondary alcohol produced by the peppermint herb, Mentha piperita, is widely used in the food and pharmaceutical industries as a cooling/soothing compound and odorant.
Here we present data of hTRPM8 collected on the Port-a-Patch using cooled solution via the External Perfusion System. Cold activated hTRPM8 was blocked by capsazepine with an IC50 in good agreement with the literature.