Fully automated data recording and analysis. 10,000 data points per day!
  • SURFE²R 96SE

    First high throughput instrument on the market for SSM-based electrophysiology
  • SURFE²R 96SE

    Finally high throughput label-free functional assays for transporters available
  • SURFE²R 96SE

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R 96SE

    Turn-key system for efficient transporter protein analysis

SURFE²R 96SE - High Throughput Transporter Research

Just like the SURFE²R N1, the SURFE²R 96SE employs SSM-based electrophysiology to resolve small currents of transporters and pumps, but elevates the throughput to the next level by parallelization of 96 measurements. The transporter samples are added to gold coated sensors in a standard 96 well-plate format. To further reduce the user's workload, all sensor coating steps can be done by the SURFE²R 96SE itself. Any substrate can be added to activate the transporter. The resulting transport or binding currents will be detected and analyzed accordingly. The whole process, from sample preparation and measurement to data analysis, is fully automated.

Features of the SURFE²R 96SE

• Recording of 96 wells in parallel using a 96-channel low-noise amplifier
• 10,000 data points per day
• Integration into the small footprint pipetting platform CyBio® Felix
• Automatization of experimental workflows including sample preparation
• Automated data analysis and export, e.g. determination of EC50
• 8 positions for different solutions (1 well to 96 well)

Features of SSM-based electrophysiology

• > 100 targets validated, 100 peer reviewed papers using SSM-based electrophysiology
• Transporters, pumps and ligand gated channels
• Measure even electroneutral exchangers and sugar binding
• Use purified membranes and vesicles from cells or proteoliposomes
• Label-free electrical measurements
• Requires only 0.1 – 1 µg protein per sensor. This is sufficient for up to 100 experiments
• Real-time data with high time resolution of 5 ms, not single point read-out
• High signal amplification compared to patch-clamp
• Fast binding kinetics can be resolved
• EC50, IC50, rate constants, comparison of transporter variants,...

The technology employs solid-supported membrane- (SSM-) based electrophysiology which was established in the late 1990s. Details about SSM-based electrophysiology are presented in the Technology section. The SURFE²R 96SE is preferred over the SURFE²R N1 when a high throughput environment is needed. This is the case, e.g. for compound or transporter screening projects. The SURFE2R 96SE is also suitable to increase throughput for basic research. Data generation is approximately 100 times faster compared to the SURFE2R N1.

For detailed information:

SURFE²R 96SE Device & Software

The SURFE2R 96SE device

The SURFE2R (surface electrogenic event reader) technology is the only available commercial solution for SSM-based electrophysiology on the market. Nanion Technologies developed the SURFE2R N1 as an easy-to-learn all-in-one device for universities and basic research and the high-throughput device SURFE2R 96SE.

The SURFE2R 96SE is integrated into the CyBio® Felix liquid handling platform. Nanion Technologies provides the electrophysiological hardware and the computer running a software package for data recording and analysis. The device can not only be used for electrophysiological measurements, but also for sample preparation and pipetting purposes. The device can measure 10,000 data points a day in a fully automated manner. In contrast to the SURFE2R N1, the SURFE2R 96SE also comes with a dedicated data analysis software which allows for automated analysis of the massive amount of data you generate each day.

The SURFE2R 96SE uses sensors in a standard 96-well plate format. Membranes containing the protein of interest are adsorbed onto these sensors. The measurement is initiated by a substrate concentration jump. The solution exchange is controlled by the liquid handling platform CyBio® Felix. The pipetting head contains 96 pipettes which load solutions from solution positions and inject them to the sensor plate. All 96 sensors of the sensor plate are measured simultaneously, but different solutions or transporters can be used for each measurement.

The sensor plate is enclosed by a Faraday cage and connected to a 96-channel amplifier as well as 96 gold pins which work as reference electrodes. The CyBio® Felix holds up to 8 well plates for 8 to 8 x 96 different solutions which allows up to 7 sequential measurements using different conditions in each automated workflow. The CyBio® pipettor allows the measurement of fast kinetics with low solution consumption, showing a time resolution of down to 5 ms by using only 50 µl of compound solution per measurement.
Software Package

Our software department developed a software package exclusively for the SURFE2R 96SE.

• The SurfControl works as the recording software and displays the data in real-time
• DataControl works as the analysis software and can be configured to automate your data analysis
• In addition, the CyBio Composer plus pre-installed workflows operate the liquid handling platform and
communicates with the recording software


SURFE²R 96SE Sensor Plates

SURFE2R 96SE Sensor Plate

The SURFE²R 96SE sensor plate is a proprietary innovative product by Nanion Technologies, developed for the SURFE²R 96SE. It is produced by an external partner, quality-assured in-house at Nanion headquarters and shipped from Munich to our international customers.

The core structure of SURFE²R 96SE sensor plates are gold-coated sensors with 3 mm diameter. This structure is incorporated into a 96 well plate for easy handling. The sensor plate is enclosed by a Faraday cage and connected to a 96-channel amplifier during the experiment.
Available plate type
  • "SURFE²R 96SE Sensor Plate": 96-well recording plate for the SURFE²R 96SE (Order # 182001)

Validated targets: SSM-based Electrophysiology

Testimonials & Case Studies

Analytik Jena - Maria Barthmes about the Cooperation on the SURFE²R Technology

Icon N1   ““We have tested several liquid-handling platforms. CyBio FeliX was the best choice for our application.”

Dr. Maria Barthmes, Product Manager SURFE²R 96SE
Nanion, Munich

Download the full case study here:

Icon N1   SURFE²R Technology Case Study (2020)   logo pdf   (1.4 MB)
Transporter Protein Research Redefined

Back to Overview

Dr. Thomas Seeger & Karin V. Niessen - Statement about the SURFE²R Technology

Icon N1   “The SURFE²R makes extremely challenging electrophysiological targets accessible to robust routine analyses. For example, we have developed an assay for investigating nicotinic acetylcholine receptor ion channels, using membranes from the Pacific electric ray, Torpedo California. In this way, we overcome many of the well-known difficulties associated with this ion channel, which allows us to efficiently obtain information regarding compound pharmacology. Obtained pharmacology values match those of patch clamp recordings exceptionally well. We find that the SURFE²R is an excellent platform for characterization of electrogenic processes in isolated membranes.”

Karin V. Niessen, Dr. Thomas Seeger
Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany

Prof. Dr. Klaus Fendler and Co-Authors - Interview about the SURFE²R Technology

Icon N1   ““SSM-based electrophysiology is a suitable technique to study bacterial ion-translocating membrane- systems which are otherwise difficult to approach using conventional electrophysiological techniques, like patch clamp.
Additionally, the mechanical stability of SSMs facilitates the transport activation through fast solution exchange, a condition that is technically challenging in a setup of a bilayer separating two solution-containing compartments.“

Dr. Miyer Fabián Patiño-Ruiz,
Faculty of Science and Engineering, University of Groningen, the Netherlands

Dr. Andre Bazzone, Application Scientist for the SURFE²R product family interviewed the authors of the publication:
"Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae"
Published 2019 in Nature Scientific Reports.

Read the publication here and download the full case study here:

Icon N1   SURFE²R Technology Customer Case Study (2020)   logo pdf   (1.3 MB)
Nature Scientific Reports: Characterization of the Na+/H+ exchanger NhaB on the SURFE²R N1

Back to Overview

Prof. Dr. Randy Stockbridge - Interview about the SURFE²R Technology

Icon N1   “We wanted to characterize the electrophysiological characteristics of our new family of transporters to find out properties such as Km and Vmax of the substrate, and ideally to screen a set of substrates for transporter activity. However, given our difficulties in developing a transport assay, I was pessimistic that we were going to be able to move beyond radiolabeled substrates.
Contrary to my expectations, electrophysiology with the SURFE²R N1 platform worked right away. We saw very robust currents in our pilot experiments during the initial training session on the instrument.“

Prof. Dr. Randy Stockbridge, Assistant Professor
University of Michigan, USA

Download the full case study here:

Icon N1   SURFE²R Technology Customer Case Study (2019)   logo pdf   (1.8 MB)
Replacing Radiolabeling Techniques with the SURFE²R N1

Back to Overview

Data and Applications

EAAT3 - Screening of 6 inhibitors

EAAT3 Surfe2r96SE data

Icon 96SE   SURFE2R 96SE data and applications:

Screening of 6 inhibitors for EAAT3. After negative control and full activation of the transporter, different concentrations of inhibitor were used. At the end the full activation was repeated.



NCX1 - High Throughput Screening on the SURFE2R 96SE


Icon N1   SURFE2R 96SE data and applications:
Cells kindly provided by Charles River.

NCX1 over-expressed in HEK cells was recorded on the SURFE2R 96SE. A membrane preparation of HEK cells expressing NCX1 was used on the SURFE2R 96SE. An activating buffer containing 100 μM Ca2+ was used to activate NCX1 (black and dark blue traces) after a negative control (0 Ca2+; light blue trace). NCX1 was activated three times after which a pharmacological experiment can be started to test for inhibitors. Wells are color-coded so that the user can get an overview of the succes of the experiment at a glance. Green wells depict resistance >500 MΩ, blue depicts wells have a resistance between 100 and 500 MΩ.

Organic Cation Transporter 2 (OCT2) - Application of Choline, TEA and Methformin


Icon 96SE   SURFE2R 96SE data and applications:

OCT2 transient currents are shown before and after the application of Choline, TEA and Methformin. A concentration-dependent activation of OCT2 could be obtained by all of the three compounds. Membranes without OCT2 (control membrane) were used as a negative control.

PepT1 - Evoked signal via Glycylglycin

Surfer N96 PepT1 full screenIcon 96SE   SURFE2R N96 (a predecessor model of SURFE²R 96SE) data and applications:

Graphical user interface of screening software used on the SURFE2R N96. Traces of PepT1 recorded from membrane preparations of PepT1 overexpressing HEK293 cells are presented. The PepT1 signal is evoked by 20mM glycylglycin.


PepT1 - Pharmacology of Losartan

Surfer N96 PepT1 Losarthan IC50 datacontrolIcon 96SE   SURFE2R N96 (a predecessor model of SURFE²R S96) data and applications:

Transport by PepT1 is inhibited by the kompetitive antagonist Losartan. When activated with 20 mM Gly-Gly, the IC50 of Losartan was 80.4 µM. Data were analysed with DataControl - the Analysis software tool for recordings on the SURFE2R N96 and SyncroPatch 96/ 384PE.

Webinars and Movies

13.10.2020 | Webinar: Employing SSM electrophysiology to capture electrogenic fluxes in membrane proteins

Icon N1  SURFE2R N1 Webinar

Date: October 13. 2020

201012 Blog Image VUM 2020


Dr. Matthias Quick (Columbia University; NY, USA)

This is an on-demand webinar from Nan]i[on and Friends 2020.

2019 - SURFE²R as a service - closing the gap between technology and application

Icon 96SE   SURFE²R 96SE Oral Presentation

Dr. Ralf Schwandner, CEO of Assay.Works GmbH, Germany
Assay.Works offers assay and screening services using the SURFE²R 96SE. Find out more here.

2018 - Pharmacological characterization of an amino acid transporter and his bacterial homologue – a case study using the SURFE²R 96SE technology

Icon 96SE   SURFE²R 96SE Oral Presentation Video

Dr. Thomas Licher, Head of in vitro Biology & High-Throughput Chemistry, Sanofi, IDD Frankfurt, Germany
Thomas made his PhD at the Center for Molecular Neurobiology in the group of Prof. Dr. Pongs on an HTS-technology for ion channel-modulating substances in 2002. He started to work for Sanofi-Aventis, later Sanofi as Post-doc, lab head and group leader in the field for Cardiac Safety and Lead Identification Technologies. Today, Thomas is Head of in vitro Biology & High-Throughput Chemistry at Sanofi in Frankfurt, Germany.

2018 - Transporters Investigated Using the SURFE²R Instruments

Icon 96SE   SURFE²R 96SE and   Icon N1   SURFE²R N1 Oral Presentation

Dr. Maria Barthmes, Product Manager SURFE²R product family, Nanion Technologies GmbH, Germany

2018 - Electrophysiological Characterization of Sugar Transporters using SSM-based Electrophysiology

Icon 96SE   SURFE²R 96SE and   Icon N1   SURFE²R N1 Oral Presentation

Dr. Andre Bazzone, Application Scientist, Nanion Technologies GmbH, Germany
Andre is an expert in the field of SSM-based electrophysiology: He made his PhD at the Max-Planck-Institute of Biophysics in Frankfurt, Germany on the electrophysiological characterization of sugar transporters using the SSM-based electrophysiology in 2016. Right afterwards, he started as Application Scientist at Nanion Technologies and today he is an important member of the Nanion SURFE²R team.


Application Notes

Product Sheets

SURFE2R 96SE - Product Sheet

Icon 96SE   SURFE²R 96SE product sheet   logo pdf   (6.8 MB)


2021 - An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE review publication in Frontiers in Pharmacology (2021)

Dvorak V., Wiedmer T., Ingles-Prieto A., Altermatt P., Batoulis H., Bärenz F., Bender E., Digles D., Dürrenberger F., Heitman L.H., IJzerman A.P., Kell D.B., Kickinger S., Körzö D., Leippe P., Licher T., Manolova V., Rizzetto R., Sassone F., Scarabottolo L., Schlessinger A., Schneider V., Sijben H.J., Steck A-L., Sundström H., Tremolada S., Wilhelm M., Wright Muelas M., Zindel D., Steppan C-M., Superti-Furga G.

2021 - A Solid Supported Membrane-Based Technology for Electrophysical Screening of B0AT1-Modulating Compounds

Icon 96SE   SURFE²R 96SE Publication in SLAS DISCOVERY: Advancing the Science of Drug Discovery. (2021)

Gerbeth-Kreul C., Pommereau A., Ruf S., Kane Jr J.L., Kuntzweiler T., Hessler G., Engel C.K., Shum P., Wei L., Czech J., Licher T.

2020 - Protein Adsorption on Solid Supported Membranes: Monitoring the Transport Activity of P-Type ATPases

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review in Molecules (2020)

Tadini-Buoninsegni F.

2018 - In vitro pharmacological characterization of the bispyridinium non-oxime compound MB327 and its 2- and 3-regioisomers

Icon 96SE  SURFE²R 96SE publication in Toxicology Letters (2018)

Niessen K.V., Seeger T. Rappenglück S., Wein T., Höfner G., Wanner K.T., Thiermanna H., Worek F.

2017 - SSM-Based Electrophysiology for Transporter Research

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE book chapter in Methody in Enzymology

Bazzone A., Barthmes M., Fendler K.

2016 - Functional analysis of Torpedo californica nicotinic acetylcholine receptors in multiple activation states by SSM-based electrophysiology

Icon 96SE   SURFE²R N96 (predecessor model of SURFE²R 96SE) publication in Toxicological Letters (2016)

Niessen K.V., Muschik S., Langguth F., Rappenglück S., Seeger T., Thiermann H., Worek F.

2018 - Drug Interactions With the Ca2+-ATPase From Sarco(Endo)Plasmic Reticulum (SERCA)

Icon 96SE   SURFE²R 96SE Review article in Frontiers in Molecular Biosciences (2018)

Tadini-Buoninsegni F., Smeazzetto S., Gualdani R., Moncelli M.R.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review article in Frontiers in Sensors (2020)

Tadini-Buoninsegni F.,Palchetti I.

2014 - Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch 96 Using Instant Frozen Cells

  Icon 96SE  SURFE²R N96 (predecessor model of SURFE²R 96SE) book chapter in Patch-Clamp Methods and Protocols

Polonchuk L.

2013 - Measuring Interference of Drug-Like Molecules with the Respiratory Chain: Toward the Early Identification of Mitochondrial Uncouplers in Lead Finding

Icon 96SE   SURFE²R N96 (predecessor model of SURFE²R 96SE) publication in Assay and Drug Development Technologies (2013)

Stock U., Matter H., Diekert K., Dörner W., Dröse S., Licher T.


2021 - Drug Effects on human Na+/Ca2+ Exchanger and Implications for Drug Development

Icon CE   FLEXcyte 96 and   Icon N1  SURFE²R N1 and   Icon 96SE   SURFE²R 96SE poster, Biophysical Society Meeting 2021  logo pdf   (1.3 MB)

2020 - A novel approach to detect electrogenic transporter activity in intact cells applied to investigate iPSC derived cardiomyocytes and neurons

 Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE poster, 64th Annual Meeting of the Biophysical Society   logo pdf   (1.6 MB)

2018 - Label-free analysis of Na+/Ca2+- exchanger (NCX) isolated from iPSC-derived cardiomyocytes

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE2R 96SE poster, Europhysiology Meeting 2018  logo pdf   (1.5 MB)

2018 - A Sensor Based Technique for Pharmacological Safety Testing of Cardiac Transport Proteins NCX, NaKATPase and Respiratory Chain Complexes

Icon CE   CardioExcyte 96 and   Icon 96SE   SUFE²R 96SE poster, SPS 2018  logo pdf   (1.7 MB)

2018 - EAAT3 Investigated using SSM-based Electrophysiology

Icon 96SE   SURFE²R 96SE poster, Biophysics Annual Meeting 2018  logo pdf   (1.2 MB)

2017 - High throughput real-time measurement of electrogenic membrane transport driven by the SLC transporters PepT1 and OcT2

Icon 96SE   SURFE²R SE96 poster, Biophysics congress 2017  logo pdf   (1.8 MB)

2017 - An emerging technique for the characterization of transport proteins: SSM-based electrophysiology

Icon N1   SURFE²R N1 and    Icon 96SE   SURFE²R SE96 poster, 19th IUPAB / 11th EBSA congress 2017  logo pdf   (3.3 MB)

2015 - Studying Electrogenic Transporters of the SLC Family in a Parallel Way Utilizing Solid Supported Membrane Technology

Icon 96SE   SURFE²R N96 poster (a predecessor model of the SURFE²R 96SE) Biophysics 2015   logo pdf   (0.8 MB)


We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.