Research Ins]i[ghts: Nanion’s Transporter Webinar Series 2022
17.11.2022 - Charge translocation reveals new insights into the transport mechanisms of human antiporters
SURFE²R 96SE

Fully automated data recording and analysis. 10,000 data points per day!
SURFE²R 96SE

First high throughput instrument on the market for SSM-based electrophysiology
SURFE²R 96SE

Finally high throughput label-free functional assays for transporters available
SURFE²R 96SE

High signal amplification compared to patch-clamp: transport & binding assays
SURFE²R 96SE

Turn-key system for efficient transporter protein analysis

Amino Acid transporter - "Secondary screening assay for amino acid transporter inhibitors on the SURFE2R 96SE"

SURFE²R 96SE application note (0.5 MB)

Summary:

Na-dependent amino acid transporters play an essential role in the uphill transport of amino acids across the plasma membrane. In this study, a novel transporter target was used for which few specific pharmacological tools are available. The aim of this study was to establish a secondary screening assay using the direct electrophysiological measurement of the transporter to identify inhibitors. Using the SURFE²R 96SE, substrate specificity was determined and inhibitors could be identified.

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SURFE²R 96SE Applications

Substrate specifity of OCT2. Three different substrates are provided in increasing concentrations cumulatively in each well. Duration 30 minutes, CHO cell membrane preparation.
Sensor stability over time. The same assay was repeated at different time intervals. The signal was stable over 30 measurements and 12 hours.
Inhibition of PepT1. IC₅₀ determination of Lys[Z(NO2)]-Val with five concentrations. One concentration per 16 wells (n=16). Duration 15 minutes, CHO cell membrane preparation.
Interface of DataControl Software. The 96 datasets can be analyzed within minutes including statistical calculations, a pdf report and the option to export the data.
Screening of 6 inhibitors for EAAC1. After negative control and full activation of the transporter, different concentrations of inhibitor were used. At the end the full activation was repeated.