• SURFE²R N1

    Easy-to-learn all-in-one device, ideal for teaching and university research
  • SURFE²R N1

    Finally label-free functional assays for transporters available
  • SURFE²R N1

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R N1

    The only instrument on the market for SSM-based electrophysiology
  • SURFE²R N1

    Turn-key system for efficient transporter protein analysis

2014 - Direct observation of electrogenic NH4(+) transport in ammonium transport (Amt) proteins

Icon N1   SURFE²R-technology (custom-built system) publication in PNAS (2014)

Authors:
Wacker T., Garcia-Celma J.J., Lewe P., Andrade S.L.

Journal:
Proceedings of the National Academy of Sciences of the United States of America (2014) 111(27):9995-10000


Significance:

We have detected and analyzed electrogenic transport of ammonium and methylammonium by members of the ammonium transport (Amt) family of membrane proteins using solid-supported membrane electrophysiology. Amt transport is pH-dependent and occurs at a rate of 30–300 ions per s per trimer, well in the range of other transport proteins. The study establishes, to our knowledge, the first in vitro assay system for Amt transport in a fully controlled setup and settles debate about whether Amt proteins function as passive ammonia channels or active ammonium transporters.

Abstract:

Ammonium transport (Amt) proteins form a ubiquitous family of integral membrane proteins that specifically shuttle ammonium across membranes. In prokaryotes, archaea, and plants, Amts are used as environmental NH4+ scavengers for uptake and assimilation of nitrogen. In the eukaryotic homologs, the Rhesus proteins, NH4+/NH3 transport is used instead in acid–base and pH homeostasis in kidney or NH4+/NH3 (and eventually CO2) detoxification in erythrocytes. Crystal structures and variant proteins are available, but the inherent challenges associated with the unambiguous identification of substrate and monitoring of transport events severely inhibit further progress in the field. Here we report a reliable in vitro assay that allows us to quantify the electrogenic capacity of Amt proteins. Using solid-supported membrane (SSM)-based electrophysiology, we have investigated the three Amt orthologs from the euryarchaeon Archaeoglobus fulgidus. Af-Amt1 and Af-Amt3 are electrogenic and transport the ammonium and methylammonium cation with high specificity. Transport is pH-dependent, with a steep decline at pH values of ∼5.0. Despite significant sequence homologies, functional differences between the three proteins became apparent. SSM electrophysiology provides a long-sought-after functional assay for the ubiquitous ammonium transporters.


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