• SURFE²R N1

    Easy-to-learn all-in-one device, ideal for teaching and university research
  • SURFE²R N1

    Finally label-free functional assays for transporters available
  • SURFE²R N1

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R N1

    The only instrument on the market for SSM-based electrophysiology
  • SURFE²R N1

    Turn-key system for efficient transporter protein analysis

2008 - Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2008)

Authors:
Wimmer F., Oberwinkler T., Bisle B., Tittor J., Oesterhelt D.

Journal:
FEBS Letters (2008) 582(27):3771-3775


Abstract:

This paper identifies the first arginine/ornithine antiporter ArcD from the domain of archea. The functional role of ArcD is demonstrated by transport assays with radioactive labelled arginine, by its necessity to enable arginine fermentation under anaerobic growth conditions and by the consumption of arginine from the medium during growth. All three experimentally observables are severely disturbed when the deletion strain ΔArcD is used. The isolated protein is verified by mass spectrometry and reconstituted in vesicles. The proteoliposomes are attached to a membrane and capacitive currents are recorded which appear upon initiation of the transport process by change from arginine‐free to arginine‐containing buffer. This clearly demonstrates that the purified 34 kD protein is the functional unit.


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