• SURFE²R N1

    Easy-to-learn all-in-one device, ideal for teaching and university research
  • SURFE²R N1

    Finally label-free functional assays for transporters available
  • SURFE²R N1

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R N1

    The only instrument on the market for SSM-based electrophysiology
  • SURFE²R N1

    Turn-key system for efficient transporter protein analysis

ClC - Inward and outward directed chloride flux

Clc Figure 1   transient currents Influx vs EffluxIcon N1   SURFE2R N1 data and applications:

According to the transport direction of 2Cl-/1H+ exchange, we observed positive (Cl- efflux) or negative (Cl- influx) transient currents.



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Background and Assay Description: 
We have analyzed transporter symmetry by comparing chloride influx with chloride efflux. In both cases, we prepared proteoliposomes in Cl-free solution. For the influx assay, we activated Clc by a single solution exchange providing 20 mM chloride. This leads to a negative current, reflecting the chloride induced net movement of one negative charge into the vesicles. For the efflux assay, we have equilibrated the sensor in solution containing 20 mM chloride, followed by Clc activation by removing chloride.

Author: Nanion Technologies, in collaboration with Merritt Maduke, Stanford University School of Medicine
Target: EriC, ClcA, ClC-ecl
Mode of transport: H+/2Cl- exchange
Organism: Escherichia coli
Sample: Proteoliposomes from purified protein, LPR 20
Platform:   Icon N1   SURFE²R N1


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