• SURFE²R N1

    Easy-to-learn all-in-one device, ideal for teaching and university research
  • SURFE²R N1

    Finally label-free functional assays for transporters available
  • SURFE²R N1

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R N1

    The only instrument on the market for SSM-based electrophysiology
  • SURFE²R N1

    Turn-key system for efficient transporter protein analysis

Clc - Proteoliposomes with different lipid-to-protein ratios

Clc Figure 4   LPRsIcon N1   SURFE2R N1 data and applications:

We tested proteoliposomes with different densities of reconstituted Clc protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.

Read more

Background and Assay Description:
We have performed a single solution exchange workflow to activate Clc by 50 mM Chloride concentration jump at pH 5.2. Each trace shown was recorded from a different sample. We have used proteoliposomes with different lipid-to-protein ratios. The higher the protein density (lower LPR), the higher the signal-to-noise ratio during the SURFE2R assay. In addition, current signals reflecting transport reactions decay faster, when the protein density is high, since more transport accelerates the charging of the membrane due to Chloride transport. As a control we performed the same assay using protein-free liposomes (red trace), which did not show any significant signal.

Author: Nanion Technologies, in collaboration with Merritt Maduke, Stanford University School of Medicine
Target: EriC, ClcA, ClC-ecl
Family / Type: Chloride Carrier/Channel Family (ClCF, TCDB: 2.A.49)
Mode of transport: H+/2Cl- exchange
Organism: Escherichia coli
Sample: Proteoliposomes from purified protein, LPR 20 – 2000 and liposomes without protein
Expression: Recombinant overexpression in E.coli
Platform:   Icon N1   SURFE²R N1


Back to Overview

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.