• SURFE²R N1

    Easy-to-learn all-in-one device, ideal for teaching and university research
  • SURFE²R N1

    Finally label-free functional assays for transporters available
  • SURFE²R N1

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R N1

    The only instrument on the market for SSM-based electrophysiology
  • SURFE²R N1

    Turn-key system for efficient transporter protein analysis

SGLT1 - EC50 for D-glucose transport

SGLT1 Figure 2   EC50 glucose

Icon N1   SURFE2R N1 data and applications:

Short Description: Na+/sugar translocation is detected using a sugar concentration jump in presence of sodium. Here we determined the KM for glucose transport by SGLT1. 
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Background and Assay Description: We performed a single solution exchange workflow by applying a D-glucose concentration jump in presence of 300 mM Na+. By application of different D-glucose concentrations in the range between 1 mM and 250 mM on the same sensor, we could determine the EC50 for D-glucose transport by SGLT1. We have used the same concentration of Mannitol as compensation in each substrate-free solution.

Author: Nanion Technologies
Target: SGLT1, SLC5A1
Family/Type: SLC5, Solute:Sodium Symporter Family (SSSF, TCDB: 2.A.21)
Mode of transport:
Na+/sugar symport
Organism: Human
Sample: Purified membrane vesicles from plasma membrane
Expression: Recombinant overexpression in CHO cells
Platform:   Icon N1   SURFE²R N1

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