• SURFE²R N1

    Easy-to-learn all-in-one device, ideal for teaching and university research
  • SURFE²R N1

    Finally label-free functional assays for transporters available
  • SURFE²R N1

    High signal amplification compared to patch-clamp: transport & binding assays
  • SURFE²R N1

    The only instrument on the market for SSM-based electrophysiology
  • SURFE²R N1

    Turn-key system for efficient transporter protein analysis

2022 - Functional investigation of GABA and Glutamate re-uptake transporters EAAT3 and GAT1 using SSM-based electrophysiology

 Icon N1   SURFE²R N1 poster, Europhysiology 2022   logo pdf   (2.48 MB)

Abstract:

GAT1 (SLC6A1) and EAAT3 (SLC1A1) are secondary active neurotransmitter transporters coupled to the ion gradients established by the NaK-ATPase. Both are expressed in neurons and play a major role in the depletion of the neurotransmitters GABA and glutamate from the synaptic cleft. Therefore, they play a major role in termination of synaptic transmission, maintaining the ambient extracellular neurotransmitter concentration and neurotransmitter recycling through reuptake. GAT1 is linked to epilepsy, schizophrenia, anxiety and ADHD. Subtype specific inhibition of EAAT3 is assumed to be beneficial during phases of insufficient energy supply by preventing reversal glutamate transport. Therefore, screening compounds to find inhibitors of these transporters is of high pharmacological interest.

We used solid-supported membrane (SSM)-based electrophysiology using the SURFE2R N1 and SURFE2R 96SE to record GAT1 or EAAT3 purified from the plasma membrane of CHO or HEK cells

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