2021 - Reliable identification of cardiac conduction abnormalities in drug discovery using automated patch clamp II: Best practices for Nav1.5 peak current in a high throughput screening environment

  SyncroPatch 384 and      Patchliner Publication in Journal of Pharmacological and Toxicological Methods (2021)

Authors:
Rotordam M.G., Obergrussberger A., Brinkwirth N., Takasuna K., Becker N., Horvátha A., Goetze T.A., Rapedius M., Furukawa H., Hasegawa Y., Oka T., Fertig N., Stoelzle-Feix S

Journal:

Journal of Pharmacological and Toxicological Methods (2021) doi:10.1016/j.vascn.2021.107125

Abstract: 

For reliable identification of cardiac safety risk, compounds should be screened for activity on cardiac ion channels in addition to hERG, including Na_V1.5 and Ca_V1.2. We identified different parameters that might affect IC₅₀s of compounds on Na_V1.5 peak and late currents recorded using automated patch clamp (APC) and suggest outlines for best practices.

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