2022 - Characterization of ion channel currents endogenously expressed in Neuro2A cells using automated patch clamp
SyncroPatch 384 poster, Europhysiology 2022 (1.01 MB)
Neuro2A cells are a mouse neuroblastoma cell line used extensively Neuro2A cells are a mouse neuroblastoma cell line used extensively to investigate neuronal differentiation, axonal growth and cell signalling pathways . They are also used as an expression system for studying ion channels. Neuro2A cells have been shown to endogenously express NaV channels, predominantly NaV1.7 but alsoNaV1.2, 1.3 and 1.4 (Lou et al., 2005), mechanosensitive channels Piezo 1 (Coste et al., 2010; Rotordam et al., 2019), purinergic receptors (Vetter & Lewis, 2010) and glutamate receptors (Van derValk & Vijverberg, 1990). In this study, we used the medium and high throughput automated patch clamp devices, Patchliner andSyncroPatch 384, to record different ion channels endogenously expressed in Neuro2A cells.
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