• SyncroPatch 384

    Next level versatility and flexibility
  • SyncroPatch 384

    True HTS and GigaOhm seals
  • SyncroPatch 384

    Your multi purpose instrument
  • SyncroPatch 384

    Powerful analysis software
  • SyncroPatch 384

    Assay flexibility via high tech
  • SyncroPatch 384

    Heating and cooling of solutions, cells and patch clamp sites

2022 - Characterization of ion channel currents endogenously expressed in Neuro2A cells using automated patch clamp

 icon sp96  SyncroPatch 384 poster, Europhysiology 2022  logo pdf   (1.01 MB)

Background:

Neuro2A cells are a mouse neuroblastoma cell line used extensively Neuro2A cells are a mouse neuroblastoma cell line used extensively to investigate neuronal differentiation, axonal growth and cell signalling pathways . They are also used as an expression system for studying ion channels. Neuro2A cells have been shown to endogenously express NaV channels, predominantly NaV1.7 but alsoNaV1.2, 1.3 and 1.4 (Lou et al., 2005), mechanosensitive channels Piezo 1 (Coste et al., 2010; Rotordam et al., 2019), purinergic receptors (Vetter & Lewis, 2010) and glutamate receptors (Van derValk & Vijverberg, 1990). In this study, we used the medium and high throughput automated patch clamp devices, Patchliner andSyncroPatch 384, to record different ion channels endogenously expressed in Neuro2A cells.

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