CaV1.2 - Current Voltage Relationship
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) data and applications:
Cells kindly provided by Charles River.
CaV1.2 expressed in CHO cells recorded on the SyncroPatch 384PE. A The screenshot shows the data acquisition and analysis software used on the SyncroPatch 384PE. The online analysis values are shown for a current-voltage experiment. B The raw traces from an example cell elicited by depolarizing steps from -60 mV to 40 mV in 10 mV increments from a holding potential of -80 mV are shown. C The normalized current-voltage plot for an average of 272 cells. A Boltzmann equation fit revealed a V0.5 of activation of -4.8 mV.