2020 - Unattended Screening Workflow in a 384-well Automated Patch Clamp System
SyncroPatch 384i poster, Safety Pharmacology Society Virtual Meeting 2020 (2.6 MB)
Within ion channel working group efforts (Fermini et al., 2015), reference compounds were tested across multiple sites and automated patch clamp (APC) platforms (Kramer et al., 2020). Resulting IC50 values were variable between drugs and platforms. In general, as Iversen et al. (2012) outlined, the quality of an assay is defined by the robustness and reproducibility of the measurement signal in the absence of any test compounds. Data robustness and reliability using an APC instrumentation should be proven carefully before an unattended full-day screening workflow is implemented.