TRPA1 - Activation by carvacrol
Activation of TRPA1 expressed in CHO cell on the SyncroPatch 384i. A Screenshot of the PatchControl 384 software depicts raw data traces of TRPA1- expressing CHO cells as recorded on one NPC-384 patch clamp chip (4 holes). A single concentration of carvacrol was added to each well and the concentration response curve calculated across the plate. B Average CRC (left) for carvacrol for n = 355 wells and average traces (right). TRPA1 was robustly activated by carvacrol. At higher concentrations (above 400 µM) desensitization of the channel was observed (tachyphylaxis) which resulted in smaller amplitudes upon repeated application.