Characterization of hClC-1 on the SyncroPatch 384 - Application Note

210426 hClC App Note Download

Summary

The voltage-gated chloride channel gene (ClC) family is highly conserved and their members are presentin both pro- and eukaryotes. In mammals, nine different ClC subtypes have been identified, which differ according to tissue distribution and subcellular location. ClC-1 is exclusively expressed in skeletal muscles.

Data and Applications

ClC-1 – Inhibition by 9-AC

ClC 1 figure Inhibition by 9 ACicon sp96   SyncroPatch 384i (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by Charles River.

Tail currents of ClC-1 expressed in CHO cells were inhibited by increasing concentration of 9-AC. A single concentration of 9-AC was added to each well and the concentration response curve constructed over multiple wells. The IC50 was calculated to be 6.3 µM for an average of 352 wells. The average current traces are also shown.

 

ClC-1 - Current-voltage plot

ClC 1 figure current voltage plot SP384 charles rivericon sp96   SyncroPatch 384i (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by Charles River.

Activation of hClC-1 tail currents expressed in CHO cells recorded on the SyncroPatch 384i (a predecessor model of SyncroPatch 384). A pre-pulse voltage step to +60 mV was followed by voltage steps from -120 mV to +80 mV for 300 ms (increasing in 20 mV steps) and the tail current was measured at the subsequent step to -100 mV. Out of a possible 384 wells, all 384 wells were used for the IV analysis

 

Publications

2019 - Structure of the human ClC-1 chloride channel

icon pap   Port-a-Patch,  icon vpp   Vesicle Prep Pro and   Icon Orbit Mini   Orbit mini publication in PLOS Biology (2019)

Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.

2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)

Authors:
Garcia-Celma J., Szydelko A., Dutzler R.

2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)

Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.

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