High Throughput Pharmacology of NaV1.9 Channels on Nanion’s SyncroPatch 384 - Application Note

210526 ICAGEN App note

Summary

The SCN11A gene encodes the voltage-gated sodium channel NaV1.9 which is predominantly expressed in small-diameter sensory neurons of dorsal root ganglia (DRG) and trigeminal ganglia.

Background Information

NaV1.9 is largely expressed in small-diameter nociceptors of the dorsal root ganglion and trigeminal ganglion neurons, but is also found in intrinsic myenteric neurons. Like Nav1.7 and Nav1.8, Nav1.9 plays a role in pain perception, having been linked to the perception of inflammatory, neuropathic, and cold-related pain. It does this primarily through its ability to lower the threshold potential of the neuron, allowing for an increase in action potential firing that leads to hyperexcitability of the neuron and increased pain perception. This channel NaV1.9 is a TTX-resistant sodium channel.

Data and Applications

NaV1.9 - Pharmacology

NaV1.9 Dataset PE 1icon sp96    SyncroPatch 384/768 PE (a predecessor model of the SyncroPatch 384) data and applications:
Cells were kindly provided by Icagen.

Screenshots of the PatchControl 384 software showing hNaV1.9 current traces in response to a voltage step protocol. Measured on the SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) using perforated patch methodology (Escin) and multi-hole chips (4 holes per well), the success rate of valuable data for the analysis was  86%. NaV1.9 is TTX insensitive. The IC50 value of lidocaine of the peak current was determined as 350 µM (Literature: 356 µM), the IC50 value of tetracaine of the peak current was determined as 12.5 µM (Literature: 32 µM).

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