210511 nanion 1026x196 03Research Ins]i[ghts: Nanion’s Transporter Webinar Series 2021

Join us over the course of a year as we dive into a series of 5 sessions (a collective total of 10 scientific presentations/talks; representing academia & industry alike) scheduled across 2021. Whether you join us from an academic or industry setting, we invite you to join us a cross the course of the year to dive deep into all things transporters. Our aim is to provide a unique platform for researchers globally to share their work and engage in meaningful active discussions. All talks will be live and accessible on-demand upon completion of the scheduled event.

02.12.2021 - Structure and Function of Na+ and H+ dependent transporters

Session Abstract: 

Na/H exchangers are a important and diverse family of secondary active transporters. These proteins are crucial in pH, sodium and volume homeostasis, dysfunction is critical in eukaryotes and procaryotes alike. In the final session of the Transporter Webinar Series David Drew and Matthias Quick share some of their research on the structure and function of this transporter family.  


Title: Structure and mechanism of the Na+/H+ exchanger NHA2

David Drew, Professor in Biochemistry,
(Stockholm University)


SLC9B2, also known as NHA2, correlates with the long-sought after sodium/lithium (Na+/Li+) exchanger linked to the pathogenesis of diabetes mellitus and essential hypertension in humans. Despite its functional importance, structural information and the molecular basis of its ion-exchange mechanism have been lacking. Here, we I briefly present the cryo EM structures of bison NHA2 in detergent and in nanodiscs at 3.0 and 3.5 Å resolution, respectively. I will then show how SSM-based electrophysiology has enabled us to conclude that NHA2 catalyses the electroneutral rather than electrogenic exchange of ions. The ion-binding site is quite distinctive, with a tryptophan-arginine-glutamate triad separated from the well-established ion-binding aspartates. These triad residues fine-tune ion binding specificity, as demonstrated by a salt-bridge swap mutant that converts NHA2 into a Li+-specific transporter.


Matthias Quick, Associate Professor of Neurobiology
(Columbia University)


When: Thursday December 2nd, 3 PM CET, 10 AM EDT

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