• Nanion Technologies: イオンチャネル研究のスマートツール

    Nanion Technologies: イオンチャネル研究のスマートツール

  • CardioExcyte 96 SOL: 心筋の光ペーシング

    CardioExcyte 96 SOL: 心筋の光ペーシング

  • SURFE²R 96SE: ラベルフリーのトランスポーターHTS

    SURFE²R 96SE: ラベルフリーのトランスポーターHTS

  • 脂質二分子膜実験: Orbitシリーズ

    脂質二分子膜実験: Orbitシリーズ

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SyncroPatch 384i

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CardioExcyte 96

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FLEXcyte 96

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Orbit 16

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Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

OmpF - "Lipid Bilayer recordings of OmpF reconstituted in Proteoliposomes "

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:   logo pdf   (1.4 MB) 

Summary:

Solvent-free planar lipid bilayers were formed in an automatic manner by bursting of giant unilamellar vesicles (GUVs) positioned by suction on the apertures of our patch clamp chips made from borosilicate glass substrate. Incubation of GUVs with purified ion channel protein of interest yielded proteoliposomes. These proteoliposomes allow for immediate recording of channel activity after GUV sealing. This approach reduces the time consuming, laborious and sometimes difficult protein reconstitution processes normally performed after bilayer formation.

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