• Nanion Technologies: イオンチャネル研究のスマートツール

    Nanion Technologies: イオンチャネル研究のスマートツール

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: ラベルフリーのトランスポーターHTS

    SURFE²R 96SE: ラベルフリーのトランスポーターHTS

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂質二分子膜実験: Orbitシリーズ

    脂質二分子膜実験: Orbitシリーズ

  • CardioExcyte 96 SOL: 心筋の光ペーシング

    CardioExcyte 96 SOL: 心筋の光ペーシング

Our Product Portfolio

SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16

Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

2018 - Quantifying Permeation of Small Charged Molecules across Channels: Electrophysiology in Small Volumes

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in ACS Omega (2018)

Authors:
Wang J., Benier L., Winterhalter M.

Journal:
ACS Omega (2018) doi: 10.1021/acsomega.8b01611


Abstract:

A major bottleneck in the development of small molecule antibiotics is to achieve good permeability across the outer membrane in Gram-negative bacteria. Optimization with respect to permeability surprisingly lacks appropriate methods. Recently we proposed to use the diffusion potential for charged molecules created by their difference in electrophoretic mobility while crossing the outer membrane channel under a concentration gradient. The latter provides semi-quantitative values but the current available setups require large volumes and thus exclude several classes of molecules. Here we propose a simple approach capturing proteoliposomes at aperture of glass surface (planar aperture or conical glass capillary) decreasing the necessary volume below 50 µL. We measured the transport of two charged molecules sulbactam and ceftazidime across the two major porins in E.coli. Both molecules permeate through these porins were observed with sulbactam owes higher permeability.


Download here

Back

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.