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Neurons - "Peri.4U and Dopa.4U stem cell-derived neurons recorded on Nanion´s Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Axiogenesis.  

Summary:

Human induced pluripotent stem cell-derived neurons (hiPSC-neurons) may provide a viable cellular model for studying the mechanisms underlying neurological diseases and drug development. Axiogenesis provides a number of hiPSC-neurons including dopaminergic neurons (Dopa.4U) and peripheral neurons (Peri.4U), amongst others. These neurons have been used on in-vitro systems such as multielectrode arrays (MEA), immunocytochemistry and calcium imaging. They are an interesting model for studying neurological diseases such as Parkinson’s Disease, as well as for efficacy, drug discovery and toxicity studies. In this study, the Patchliner was used to record from Dopa.4U and Peri.4U neurons in voltage and current clamp modes. The cell harvesting procedure was optimized (using papain) to ensure that the cells retained proximal dendrites and initial axon segments in order to maintain ion channel expression present in these regions. Due to their irregular shape (presence of processes), success rate (typically 20 - 50% for RSeal >200 MΩ) was lower than other cell types which have a smooth, round shape, e.g. standard cell lines. Voltage-gated Na+ (NaV) and K+ (KV) currents were recorded in both cell types. Action potentials (AP) were also recorded and block of the AP of Dopa.4U cells by lidocaine is shown.

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