KV1.3 - "Pharmacological analysis of heterologous and endogenous expressed KV1.3 channels in Sf21 insect cells and T-lymphocytes"
Patchliner application note: (0.7 MB)
Cells were kindly provided by conoGenetix.
The voltage-gated potassium channel KV1.3 is expressed in human naive, central memory T cells (TCM), and effector memory T cells (TEM). TEM cells are important mediators of a variety of chronic inflammatory autoimmune diseases including multiple sclerosis (MS), rheumatoid arthritis (RA), psoriasis, and vasculitis. Antigenic stimulation of TEM cells results in upregulated KV1.3 channel expression. It is proposed that a selective block of KV1.3 channels leads to an inhibition of functionality of TEM cells without affecting the function of naive and TCM cells. Thus, KV1.3 is a promising therapeutic target that could be used to efficiently medicate chronic autoimmune diseases without generating typical side effects associated with today’s immunosuppressive therapies. In the laboratory of conoGenetix biosciences the insect cell line Spodoptera frugiperda (Sf21) is used in combination with a Baculovirus infection system for the heterologous expression of recombinant ion channels. Sf21 cells offer highly specific protein expression and correct posttranslational modifications resulting in functional channels with comparable electrophysiological parameters of ion channels in vivo. In this Application Note we compare the electrophysiological properties of the potassium selective ion channel KV1.3 expressed in Sf21 cells and endogenously expressed KV1.3 channels in TEM cells. For the pharmacological analysis the 4 - channel Nanion Patchliner and one of our innovative, KV1.3 specific, proprietary peptides was used.