TRPV3 - "Heat activation of TRPV3 on Nanion's Patchliner"
Patchliner application note: (0.6 MB)
The transient receptor potential cation channel, subfamily V, member 3 (TRPV3), is a ligand-gated, nonselective cation channel first described in 2002. It exhibits 43% sequence identity to TRPV1. Although TRPV3 has been detected immunologically in the CNS and suggested to be often co-localized with TRPV1, it is found more robustly in keratinocytes in skin and, given it’s threshold for temperature activation of >34˚C, it has been speculated that TRPV3 may act in co-operation with sensory afferents to perceive warmth and signal elevated temperature. TRPV3 can also be activated by the ligand 2-Aminoethoxydiphenyl borate (2-APB). The TRPV3 channel, along with other TRPV channels, may play an important role in chronic pain and, therefore, is receiving increasing attention as a potential therapeutic candidate for the treatment of chronic pain. Here we present data collected on a 4-channel Patchliner with temperature control showing the potential use of the Patchliner to record TRPV3 currents activated by 2-APB or heat. As previously reported, TRPV3 currents sensitize to repeated applications of 2-APB or heat, a phenomenon we also observed. At low concentrations of 2-APB, the currents were primarily outwardly rectifying but at higher concentrations and with prolonged exposure they often became dual rectifying (data not shown). This is also in good agreement with the literature. In contrast, the temperature-activated responses were always outwardly rectifying with little inward current. The inward currents activated by 2-APB could be blocked by ruthenium red (RR) as expected.