• Nanion Technologies: イオンチャネル研究のスマートツール

    Nanion Technologies: イオンチャネル研究のスマートツール

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: ラベルフリーのトランスポーターHTS

    SURFE²R 96SE: ラベルフリーのトランスポーターHTS

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂質二分子膜実験: Orbitシリーズ

    脂質二分子膜実験: Orbitシリーズ

  • CardioExcyte 96 SOL: 心筋の光ペーシング

    CardioExcyte 96 SOL: 心筋の光ペーシング

Our Product Portfolio

SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

2017 - lnvestigation of the Ion Channels hTMEM16A/Ano1 and TRPC5 and their Modulation by Intracellular Calcium

icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, BPS Meeting 2017  logo pdf   (1.3 MB)

Abstract:

Targets:
hTMEM16A/Anoctamin1 is a Ca2+ activated Cl- channel (CACC) which has a broad functional spectrum in processes including trans-epithelial ion transport, olfaction, photo-transduction, smooth muscle contraction, nociception, cell proliferation and control of neuronal excitability. The ion channel has been implicated to play a role in a number of health disorders and may be an important therapeutic target in cystic fibrosis, asthma, pain and some human cancers.
hTRPC5 is a non-selective cation channel expressed in many areas of the brain particularly the hippocampus, amygdala and cerebellum, amongst others. Although the physiological and patho-physiological role of hTRPC5 is not fully known, it does appear to be important in neuronal function, in particular during development where it is involved in hippocampal neurite outgrowth and growth cone morphology. Knockout mouse studies have also revealed that TRPC5 plays an essential role in innate fear. hTRPC5 is expressed in some regions outside of the CNS including the heart where it contributes to cardiac hypertrophy in heart failure.

Challenge:
hTMEM16A and hTRPC5 are activated by elevated cytosolic Ca2+ concentrations. To initiate channel activity intracellular solution must be exchanged from a Ca2+ free solution to a solution containing Ca2+. Some of the common instruments for automated patch clamp are limited to do an internal exchange at all. But also for the other devices it is challenging because they rely on fluoride in the internal solution to perform proper seals. Unfortunately fluoride has a strong tendency to precipitate with calcium, which makes it impossible to correctly adjust a precise Ca2+ concentration needed for channel activation. Furthermore current run-down or desensitization are common problems associated with recording hTMEM16A7 and hTRPC510.

Accomplishment:
On the SyncroPatch 384PE we developed two very reliable assays for hTMEM16A and hTRPC5 in the presence of fluoride-free intracellular solutions using the internal perfusion feature. The presented data show that the intracellular solution can be exchanged in a very robust manner to investigate Ca2+ sensitivity. Besides voltage dependence and pharmacology characteristics of hTMEM16A and hTRPC5 are presented on this poster. The hTMEM16A assay was established using the PE/Felix wheatear the hTRPC5 assay was run on the PE/Biomek.

Back

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.