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2018 - Development of an on-chip antibiotic permeability assay with single molecule detection capability

icon vpp   Vesicle Prep Pro publication in IEEE Transactions on NanoBioscience (2018)

Authors:
Guzel F.D., Citak F.

Journal:
IEEE Transactions on NanoBioscience (2018) 99:1-1


Abstract:

Electrophysiology is the method of choice to characterize membrane channels. In this study, we demonstrate a patch pipette based simple miniaturization that allows performing conductance measurements on a planar lipid bilayer in a microfluidic channel. Membrane proteins were reconstituted into Giant Unilamellar Vesicles (GUVs) by electroswelling, and GUVs with a single channel insertion were patched at the tip of pipette. We applied this approach to investigate the interactions of porins from E. coli with single antibiotics, and this will potentially provide information on the permeability rates. The results of this study suggest that this approach can be extended to the integration of several pipettes into the microfluidic channel from different positions, allowing the multiplexed recordings and also reducing the substrate consumption below μL volumes.


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