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    SyncroPatch 384i: HTS Automated Patch Clamp

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    SURFE²R 96SE: ラベルフリーのトランスポーターHTS

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    Dynamic Clamp: Patchliner

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    脂質二分子膜実験: Orbitシリーズ

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    CardioExcyte 96 SOL: 心筋の光ペーシング

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2020 - Reliable identification of cardiac liability in drug discovery using automated patch clamp: Benchmarking best practices and calibration standards for improved proarrhythmic assessment

icon pl   Patchliner and   icon sp96   SyncroPatch 384i (SyncroPatch 384PEa predecessor model) publication in the Journal of Pharmacological and Toxicological Methods (2020)

Authors:
Brinkwirth N., Takasuna K., Masafumi D., Becker N., Obergrussberger A., Friis S., Furukawa Y., Hasegawa Y., Oka T., Ohtsuki A., Fertig N., Stoelzle-Feix S.

Journal:

Journal of Pharmacological and Toxicological Methods (2020) doi: 10.1016/j.vascn.2020.106884


Introduction: 

Screening compounds for activity on the hERG channel using patch clamp is a crucial part of safety testing. Automated patch clamp (APC) is becoming widely accepted as an alternative to manual patch clamp in order to increase throughput whilst maintaining data quality. In order to standardize APC experiments, we have investigated the effects on IC50 values under different conditions using several devices across multiple sites.

Methods: 

APC instruments SyncroPatch 384i, SyncroPatch 384PE and Patchliner, were used to record hERG expressed in HEK or CHO cells. Up to 27 CiPA compounds were used to investigate effects of voltage protocol, incubation time, labware and time between compound preparation and experiment on IC50 values.


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