ChR2

ChR2 - Variation of light intensity

  SURFE²R N1 data and applications:

We observed saturation kinetics for the light-induced ChR2 activity with increasing LED intensities using a prototypical SURFE²R N1 add-on for activation by light.

Read more

ChR2 - Symmetry of transport

  SURFE²R N1 data and applications:

Depending on the direction of the Na⁺ gradient, we observed positive or negative transient currents after ChR2 activation by light. A negative control without ChR2 shows no current signal upon light activation.

Read more

ChR2 - Stability of Retinal binding

  SURFE²R N1 data and applications:

We investigated the effects of retinal addition and removal on light-induced ChR2 activity using a prototypical SURFE²R N1 add-on for activation by light.

Read more

ChR2 - Membrane potential as driving force

  SURFE²R N1 data and applications:

We investigated the effect of a membrane potential - generated by Na⁺/Ca²⁺ exchange activity of NCX1 - on the signal amplitude of light induced Na⁺ flux by ChR2.

Read more

ChR2 - Exposure time and frequency of light

  SURFE²R N1 data and applications:

We investigated the influence of the duration and frequency of the light pulses for ChR2 activation on the stability of the ChR2 signals over time.

Read more

ChR2 - Cell-based assay optimization

  SURFE²R N1 data and applications:

For assay optimization we checked the influence on cell suspension volume we have attached to one single sensor on the signal-to-noise ratio during the SURFE²R experiment.

Read more

Cardiomyocytes - Optogenetics meets cardiac safety

   CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

The stimulating optical lid, CardioExcyte 96 SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2).

Right graph: LPM – Light pulse per minute plotted against the recorded beat rate (average of 96 wells). ChR2 transfected Cor.4U cells are following the optical pace rate.

Cardiomyocytes - Myocyte phase II study: CiPA conform analysis and arrhythmia detection

   CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

Nanion developed a CiPA conform analysis for the Myocyte phase II study. The feature comes along is included in our CiPA analysis routine. Automated arrhythmia detection is just one highlight out of many when it comes to the CardioExcyte 96 software.

Cardiomyocytes - Channelrhodopsin 2 (ChR2) transfected Cor.4U cells and optical pacing

   CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.

ChR2 transfected Cor.4U cells are following the optical pace rate.

Raw data traces upon a 1 Hz, 1.5 Hz, 2 Hz., 2.5 Hz and 3 Hz stimulation rate, extracellular field potentials (top) and impedance (bottom).

2020 - Wireless Charging Electrochemiluminescence System for Ionic Channel Manipulation in Living Cells

   Port-a-Patch publication in ACS Applied Materials & Interfaces (2020)

Authors:
Peng K., Liu S., Lv F., Fu X., Hussain S., Zhao H., Liu L., Wang S.

2018 - An update on the advancing high-throughput screening techniques for patch clamp-based ion channel screens: implications for drug discovery

   SyncroPatch 384/768PE (a predecessor model of SyncroPatch 384/768i) and      Patchliner publication in Expert Opinion on Drug Discovery

Authors: 
Obergrussberger A., Goetze T.A., Brinkwirth N., Becker N., Friis S., Rapedius M., Haarmann C., Rinke-Weiß I., Stölzle-Feix S., Brüggemann A., George M., Fertig N.

2017 - Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation

  CardioExcyte 96 publication in International Journal of Molecular Sciences

Authors:
Rehnelt S., Malan D., Juhasz K., Wolters B., Doerr L., Beckler M., Kettenhofen R., Bohlen H., Bruegmann T., Sasse P.