FoF1 - ATP phosphohydrolase (H+-Transporting) - ATP-Synthase
Family:
F-type ATPases, members belong to the P-bond hydrolysis driven transporters superfamily
Assembly:
The F1 motor, responsible for ATP turnover, has the subunit composition α3β3γδε.The F0 motor, responsible for ion translocation, is complex in mammals, with probably nine subunits centring on A, B, and C subunits in the membrane, together with D, E, F2, F6, G2 and 8 subunits. Multiple pseudogenes for the F0 motor proteins have been defined in the human genome.
Function:
F-type ATPases are found in eukaryotic mitochondria and chloroplasts as well as in bacteria. The F-type ATPase in humans is a mitochondrial membrane-associated multimeric complex consisting of two domains, an F0 channel domain in the membrane and an F1 domain extending into the lumen. Proton transport across the inner mitochondrial membrane is used to drive the synthesis of ATP, although it is also possible for the enzyme to function as an ATPase.
Data and Applications
Respiratory Chain Complex V - Action of the ATP Synthase after Application of ATP
SURFE2R N1 data and applications:
The Respiratory Chain Complex V uses the transmembrane proton gradient (produced by Complex I, III and IV) to generates ATP from ADP plus phosphate. One component of ATP synthase acts as an ion channel that provides for a proton flux back into the mitochondrial matrix. This reflux releases free energy, which is used to drive ATP synthesis, catalyzed by the other component of the complex. Experiments on the SURFE2R N1 show the action of the ATPase after application of ATP.
Posters
2017 - High throughput real-time measurement of electrogenic membrane transport driven by the SLC transporters PepT1 and OcT2
SURFE²R SE96 poster, Biophysics congress 2017 
(1.8 MB)
Publications
2019 - Arg-8 of yeast subunit e contributes to the stability of F-ATP synthase dimersand to the generation of the full-conductance megachannel
Orbit mini publication in Journal of Biological Chemistry (2019)
Authors:
Guo L., Carraro M., Carrer A., Minervini G., Urbani A., Masgras I., Tosatto S.C.E., Szabò I., Bernardi P., Lippe G.
2013 - Measuring Interference of Drug-Like Molecules with the Respiratory Chain: Toward the Early Identification of Mitochondrial Uncouplers in Lead Finding
SURFE²R N96 (predecessor model of SURFE²R 96SE) publication in Assay and Drug Development Technologies (2013)
Authors:
Stock U., Matter H., Diekert K., Dörner W., Dröse S., Licher T.
2010 - Electrophysiology of respiratory chain complexes and the ADP-ATP exchanger in native mitochondrial membranes
SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2010)
Authors:
Watzke N., Diekert K., Obrdlik P.
2003 - Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus
SURFE²R-technology (custom-built system) publication in Biophysical Journal (2003)
Authors:
Burzik C., Kaim G., Dimroth P., Bamberg E., Fendler K.
a. P-bond hydrolysis driven transporters