• NR1/NR2A

    NR1/NR2A recorded on the SyncroPatch 384PE

NR2A | GluN2A | Glutamate Receptor Ionotropic, NMDA 2A

Glutamate Receptors

The Glutamate receptor family has further been divided into ionotropic and metabotropic glutamate receptors. 
The metabotropic glutamate receptor subfamily contains 8 members (mGluR1 - mGluR8)
The ionotropic glutamate receptors subfamily contains 16 members: 
AMPA receptors: GluA1 (GluR1) - GluA4 (GluR4)
Kainate receptors: GluK1 (GluR5), GluK2 (GluR6), GluK3 (GluR7) , GluK4 (KA-1), GluK5 (KA-2)
NMDA receptors: GluN1 (NR1), GluN2A (NR2A), GluN2B (NR2B), GluN2C (NR2C), GluN2D (NR2D), GluN3A (NR3A), GluN3B (NR3B)

Regulation and Function:
Ionotropic glutamate receptors are ligand-gated nonselective cation channels that allow the flow of K+, Na+ and sometimes Ca2+ in response to glutamate binding. Metabotropic glutamate receptors belong to the subfamily C of G protein-coupled receptors. Glutamate receptors are responsible for the glutamate-mediated postsynaptic excitation of neural cells, and are important for neural communication, memory formation, learning, and regulation.

Data and Applications

NMDA NR1/NR2A - Activation and Modulation

NMDA 384well view

icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) data and applications:
Cells were kindly provided by B'Sys.

Here, activation and modulation of the NR1/NR2A subunit containing NMDA receptors are shown. Currents were evoked by application of 10 µM Glutamate in the presence of 10 µM Glycine.

Application of the neurosteroid Pregnenolone sulfate (PS) potentiates the current response induced by Glutamate. The potency of PS was analysed in a single-point screen. The EC50 of PS (39.6 µM), comprising 74% of the cells is nicely corresponding to litereature values (Irwin, et al. Neurosci. Lett. 1992)


2017 - Pharmacological Characterization of the NMDA A-B-C by Automated Patch Clamp

icon sp96   SyncroPatch 384PE (a predecessor model of SyncroPatch 384) poster, BPS Meeting 2017  logo pdf   (4.9 MB)

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