Na+/K+-ATPase - Sodium/Potassium-Exchanging ATPase

Family:
P-type ATPase (P-ATPase) Superfamily

Subgroups and Members:
P-type ATPases can be divided into five subfamilies (types)
Type I consists of the transition/heavy metal ATPases.
Type II ATPases (specific for Na⁺,K⁺, H⁺ Ca²⁺, Mg²⁺ and phospholipids) predominate in eukaryotes. SERCA and Na⁺/K⁺ ATPases are member of this group.
There are 3 major paralogs, SERCA1-3, which are expressed at various levels in different cell types: SERCA1 - SERCA3, encoded by the genes ATP2A1 - ATP2A3.
Type III ATPases contains the plasma membrane H⁺-ATPases from plants and fungi.
Type IV ATPases have been shown to be involved in the transport of phospholipids
Type V ATPases have unknown specificity.

Topology:
Many of these protein complexes are multisubunit with a large subunit serving the primary ATPase and ion translocation functions. Many eukaryotic P-type ATPases are monomeric or homodimeric enzymes of the catalytic subunit that hydrolyzes ATP. They contain the aspartyl phosphorylation site and catalyzes ion transport. The Na+/K+-ATPases, the Ca²⁺-ATPases and the (fungal) H⁺-ATPases of higher organisms exhibit 10 transmembrane α helical spanners (TMSs).

Function:
P-type ATPases are α-helical bundle primary transporters named based upon their ability to catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump and their energy source, adenosine triphosphate (ATP). Most members of this transporter superfamily catalyze cation uptake and/or efflux, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.

Data and Applications

Sodium-Potassium ATPase - Analysis

Icon N1 SURFE²R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na⁺/K⁺ pump is responsible for the active transport of Na⁺ and K⁺ in the cells containing relatively high concentrations of K⁺ ions but low concentrations of Na⁺ ions. The Na⁺/K⁺-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na⁺ and K⁺, or in the presence of ATP and Na⁺.

Posters

2018 - A Sensor Based Technique for Pharmacological Safety Testing of Cardiac Transport Proteins NCX, NaKATPase and Respiratory Chain Complexes

Icon CE CardioExcyte 96 and Icon 96SE SUFE²R 96SE poster, SPS 2018 logo pdf (1.7 MB)

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2017 - An emerging technique for the characterization of transport proteins: SSM-based electrophysiology

Icon N1 SURFE²R N1 and Icon 96SE SURFE²R SE96 poster, 19th IUPAB / 11th EBSA congress 2017 logo pdf (3.3 MB)

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Publications

2021 - Structural basis for Potassium transport by KdpFABC

Icon N1 SURFE²R N1 pre-print in bioRxiv (2021)

Authors:

Sweet M.E., Larsen C., Zhang X., Schlame M., Pedersen B.P., Stokes D.L.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Icon 96SE SURFE²R 96SE and Icon N1 SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors:
Tadini-Buoninsegni F.,Palchetti I.

2017 - Identification of Na+/K+-ATPase inhibition-independent proarrhythmic ionic mechanisms of cardiac glycosides

Patchliner publication in Nature Scientific Reports (2017)

Authors:
Koh C.H., Wu J., Chung Y.Y., Liu Z., Zhang R.R., Chong K., Korzh V., Ting S., Oh S., Shim W., Tian H.Y., Wei H.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

Icon N1 SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Cytotoxic effect of zinc oxide nanoparticles on murine photoreceptor cells via potassium channel block and Na+/K+‐ATPase inhibition

Port-a-Patch publication in Cell Proliferation (2017)

Authors:
Chen C. Bu W., Ding H., Li Q., Wang D., Bi H., Guo D.

1999 - Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

Icon N1 SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na/K-ATPase Investigated on Solid Supported Membranes: Rapid Solution Exchange with a New Technique

Icon N1 SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K.

1993 - Charge transport by ion translocating membrane proteins on solid supported membranes

Icon N1 SURFE²R-technology (custom-built system) publication in Biophysical Journal (1993)

Authors:
Seifert K., Fendler K., Bamberg E.

a. P-bond hydrolysis driven transporters

Na⁺/K⁺-ATPase