NaV1.3 | sodium voltage-gated channel alpha subunit 3
Family:
Sodium channels
Subgroups:
NaV1.1-1.9
Topology:
Alpha subunits consist of four homologous domains (I-IV) with six transmembrane alpha helices (S1–S6) and a pore-forming loop. One a subunit may associate with 1 or 2 b subunits to make up the channel.
NaV1.3: Background Information
NaV1.3 is abundantly expressed in neuronal tissues during embryonic and neonatal stages of development and is rare in adult tissues. After axonal transection, NaV1.3 is upregulated in dorsal root ganglia (DRG) neurons adding to the evidence that upregulation of NaV1.3 may play a role in rendering axotomized DRG neurons hyperexcitable, thus contributing to neuropathic pain. It is thought that the fast activation and inactivation kinetics of NaV1.3, together with its rapid repriming kinetics and persistent current component, contributes to the development of spontaneous ectopic discharges and sustained rates of firing characteristics of injured sensory nerves. NaV1.3 is TTX sensitive.
Gene:
SCN3A
Human Protein:
UniProt Q9NY46
Tissue:
Brain neurons, peripheral neurons, injured neurons, spinal cord, heart
Function/ Application:
Excitability of neurons, generation of action potentials
Pathology:
Familial autism, deafness, epilepsy, pain
Interaction:
Contactin, β1 subunit, β3 subunit, β8 subunit, calmodulin, LRP, C1D
Modulator:
Tetrodotoxin, AFT-II, Vinpocetine, Flecainide, Lidocaine
Assays:
Patch Clamp: whole cell, room temperature, State- and use-dependence
Particularities:
NaV channel analysis requires GigaOhm seals and a stable and low access resistance
Recommended Reviews:
International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005
Publications
2017 - High-throughput electrophysiological assays for voltage gated ion channels using SyncroPatch 768PE
SyncroPatch 768PE (a predecessor model of SyncroPatch 384/768i) publication in PLoS One (2017)
Authors:
Li T, Lu G, Chiang E.Y., Chernov-Rogan T., Grogan J.L., Chen J.
2012 - Isolation, characterization and total regioselective synthesis of the novel μO-conotoxin MfVIA from Conus magnificus that targets voltage-gated sodium channels
Port-a-Patch publication in Biochemical Pharmacology (2012)
Authors:
Vetter I., Dekan Z., Knapp O., Adams D.J., Alewood P.F., Lewis R.J.
2010 - Propranolol blocks cardiac and neuronal voltage-gated sodium channels
Patchliner publication in Frontiers in Pharmacology (2010)
Authors:
Wang D.W., Mistry A.M., Kahlig K.M., Kearney J.A, Xiang J., George Jr. A.L.