NCX_Mj - Sodium-Calcium Exchanger - Uncharacterized membrane protein MJ0091

Calcium-Cation Antiporter family (CaCA)

The CaCA superfamily is composed of five families: K+-independent Na+/Ca2+ exchangers (NCXs), cation/Ca2+ exchangers (CCXs), YbrG transporters and cation exchangers (CAXs). Phylogenetic and alignment studies indicate that one of the mammalian NCKXs, NCKX6, and its related proteins form a unique group, designated cation/Ca2+ exchangers (CCXs)

Proteins of the CaCA family are found ubiquitously, having been identified in animals, plants, yeast, archaea and divergent bacteria. They exhibit widely divergent sequences, and several have been shown to have arisen by a tandem intragenic duplication event. The most conserved portions of this repeat element, α1 and α2, are found in putative transmembrane regions 2-3 and transmembrane regions 7-8. Members of this family contain nine transmembrane regions in mammals.

All of the characterized animal proteins catalyze Ca2+:Na+ exchange although some also transport K+. The NCX plasma membrane proteins exchange 3 Na+ for 1 Ca2+. Mammalian Na2+/Ca2+ exchangers exist as three isoforms NCX1-3 which are about 70% identical to each other.

Data and Applications

NCX_Mj - EC50 for Calcium and Magnesium

F1 2Icon N1   SURFE2R N1 data and applications:

Measurement of the transport activity of NCX_Mj. The first solution exchange provides a Na+-free solution to establish an outward directed Na+ gradient; the second solution exchange provides the divalent cation to activate the ion exchanger. By applying different concentrations of one divalent cation on the same sensor, we determined EC50 for Ca2+, Mg2+ and Sr2+.

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2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

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