2015 - Functional Characterization of Prokaryotic NCX by Solid Supported Membrane Technology

Icon N1   SURFE²R N1 poster, Gordon Research Conference 2015  logo pdf   (0.9 MB)


Sodium/calcium exchangers (NCXs) are membrane transporters which play an important role in Ca2+ homeostasis and Ca2+ signaling. These proteins have been implicated in various Ca2+ dependent physiological processes such as neurotransmission, skeletal and smooth muscle contraction, cardiac contractility and apoptosis. Here, we use solid supported membrane (SSM) technology to performed functional analysis of NCX_Mj, an archaeal NCX isoform which has recently been crystalized. Using this approach we characterized the substrate affinity, ion specificity and inhibition by divalent cations of this archaeal exchanger protein. In conclusion we found a high functional similarity of
NCX_Mj and eukaryotic NCX isoforms, although the prokaryotic protein lacks a large regulatory domain. Furthermore our results demonstrate that SSM based technology is well suited for state-of-the-art functional characterization of transporter proteins.


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